基于萘酰亚胺的H_(2)S可视化荧光探针构建

Construction of a Visual H_(2)S Fluorescent Probe Based on Naphthalimide

硫化氢(H_(2)S)在生物体中具有重要的调控作用,但其具有剧毒,一旦浓度超出生理水平,会对呼吸系统、神经系统及心脏器官造成损伤。基于1,8-萘酰亚胺设计合成了一种对H_(2)S敏感的荧光探针DHBID。研究发现,随着H_(2)S含量的增加,该探针在480 nm处荧光强度显著增强,信背比可达15倍。以Na_(2)S作为H_(2)S供体,当Na_(2)S浓度在0~250 nmol/L范围内,该探针具有明显的响应信号,且在5~100 nmol/L的范围内荧光强度与Na_(2)S浓度呈现良好的线性关系Y=19.258X+319.460(R^(2)=0.9758),检测下限为2 nmol/L。DHBID探针对H_(2)S敏感,反应时间相对较短(30 min即可完成),并具有高选择性,对常见的阴离子无响应。此外,在响应范围内,随着H_(2)S含量的增加,反应溶液颜色逐渐由淡黄色变成玫红色,从而实现对不同浓度H_(2)S的可视化检测。

Hydrogen sulfide(H_(2)S)plays an important regulatory role in organisms.However,it is highly toxic,and once the concentration exceeds physiological levels,it can damage the respiratory system,nervous system and even heart organs.A H_(2)S-sensitive fluorescent probe DHBID based on 1,8-naphthimide was designed and synthesized.Experimental results showed that fluorescence intensity at 480 nm was significantly enhanced with the increase in Na_(2)S concentration,with the signal-to-background ratio up to 15 times.With the Na_(2)S used as H_(2)S donor,the probe DHBID showed an obvious response signal to H_(2)S in the range of 0~250 nmol/L,and had a good linear relationship in the concentration range of 5~100 nmol/L with its correlation equation expressed as Y=19.258X+319.460(R^(2)=0.9758),with the detection limit of 2 nmol/L.The probe was highly sensitive to H_(2)S with a relatively short reaction time,and highly selective with no response to common anions.Besides,within response range,with the increase in H_(2)S concentration,the color of the reaction solution gradually changed from light yellow to rose red,which could realize the visual detection of H_(2)S with different concentrations.