葡萄叶面肥富硒特性及可溶态硒分布研究

Research of selenium enrichment characteristic by foliar fertilization and soluble speciation analysis in grape

探讨了叶面喷施氨基酸硒肥对富硒夏黑葡萄的应用效果,考察其富硒特性及可溶态硒的分布规律。结果表明,富硒最佳硒液浓度为250 mg/kg,效果明显;从夏黑葡萄生长绽叶期就开始定期喷施叶面硒肥,可显著改善叶片质量,有助于葡萄有机态硒的富集,葡萄硒含量最高达138μg/kg;葡萄富硒时期与富硒效果呈正相关关系(R^(2)=0.959 4),叶面肥富硒效果显著。进一步考察富硒葡萄中不同可溶态硒(碱溶态、酸溶态、Tris-HCl态、水溶态)在葡萄中的分布规律,结果表明碱态硒的含量最优,在40℃、80min下有最好的提取率。

The application effect of foliar spraying amino acid selenium fertilizer on selenium rich Summer Black grape was studied,and the characteristics of selenium enrichment and the distribution of selenium forms were investigated. The results showed that the best concentration of selenium solution was 250 mg/kg,and the effect was obvious;Spraying selenium fertilizer regularly from the growth and blooming stage of Summer Black grape could significantly improve the quality of leaves and help to enrich organic selenium,with the highest selenium content of 138 μg/kg. There was a positive correlation between the period of selenium enrichment and the effect of selenium enrichment(R^(2)=0.959 4),the selenium enrichment effect was remarkable. The change and distribution of different forms of selenium(alkali soluble,acid soluble,Tris-HCl state,water-soluble)in selenium rich grapes were further investigated. The results showed that the extraction rate of alkaline selenium was the best at 40 ℃ and 80 min.