自然罹病死亡云斑白条天牛幼虫体内微生物的分离鉴定

Isolation and identification of microorganism in larvae of naturally dead Batocera lineolata

为了找到对云斑白条天牛(Batocera lineolata)具有杀虫活性的生防菌,开发云斑白条天牛生物防治的高效菌剂,采用传统的微生物分离培养与16S rDNA基因测序相结合的方法对自然罹病死亡的云斑白条天牛幼虫体内微生物进行分离鉴定。结果表明,获得的21株细菌分别属于克雷伯氏菌属(Klebsiella)、沙雷氏菌属(Serratia)和产碱杆菌属(Alcaligenes)等12属;获得的8株真菌分别属于虫草属(Cordyceps)、螺旋聚孢霉属(Clonostachys)、镰刀菌属(Fusariums)等6个属。讨论了沙雷氏菌属、虫草属、螺旋聚孢霉属等菌属的杀虫活性及其开发利用潜力。

In order to find biocontrol bacteria with insecticidal activity against Batocera lineolata,and develop efficient microbial agents for biological control of Batocera lineolata,the traditional method of microbial isolation and culture combined with 16S rDNA gene sequencing was used to isolate and identify the microorganisms in the larvae of Batocera lineolata. The results showed that 21 strains of bacteria were classified into 12 genera including Klebsiella,Serratia and Alcaligenes,respectively. The 8 strains of fungi are classified into 6 genera including Clonostachys,Fusariums and Cordyceps. The insecticidal activities and exploitation potential of Serratia,Cordyceps and Clonostachys were discussed.