纳米抗体特性及其在农产品真菌毒素检测中的应用

Nanobody characteristics and its important applications for determination of mycotoxins in agricultural products

纳米抗体是由骆驼科动物产生的一类天然缺失轻链的重链抗体可变区。相比较于传统单克隆抗体和多克隆抗体,其具有体积小、热稳定性强、耐有机溶剂、折叠性可逆、易于表达、高亲和力、可特异性识别独特表位等天然优势。因此,纳米抗体在生物和农业等研究领域备受关注。本文综述了纳米抗体的结构、生化特性以及在农产品真菌毒素检测中的研究进展,并对纳米抗体在农产品真菌毒素检测方向的发展前景进行了展望。

Nanobodies are a class of naturally deficient light chain of heavy chain antibodies produced by camelid bodies.Compared with traditional monoclonal antibodies and polyclonal antibodies,they have the advantages of small size,excellent thermal stability,high organic solvent tolerance,reversible foldability,easy expression,high affinity and specific recognition of unique epitopes.Therefore,nanobodies have attracted much attention in the fields of biology and agriculture.This article reviewed the structure,biochemical characteristics and the current research status in the field of agricultural mycotoxin detection of nanobodies,and prospects for the development prospects of nanobodies in the detection of mycotoxins in the agricultural products.