职业性矽肺患者肺泡巨噬细胞中铁蛋白轻链蛋白表达分析

Analysis on protein expression of ferritin light chain in alveolar macrophages of patients with occupational silicosis

目的 观察不同期别职业性矽肺(以下简称“矽肺”)患者肺泡巨噬细胞(AM)中铁蛋白轻链(FTL)的蛋白表达规律。方法 采用判断抽样方法,选择矽肺壹期、贰期和叁期男性患者各15例设为矽肺壹期、贰期和叁期组,同时以15例肺部存在小阴影但尚未能诊断为矽肺壹期的男性矽尘作业工人为对照组。收集4组人群的支气管肺泡灌洗液,分离、纯化AM,经裂解后提取蛋白。采用蛋白质印迹法检测AM中FTL蛋白的相对表达水平。结果 矽肺壹期、贰期和叁期组患者AM中的FTL的蛋白相对表达水平均低于对照组(P值均<0.05);且随着矽肺期别的升高,AM中FTL蛋白相对表达水平下降(P<0.05)。结论 不同期别矽肺患者AM中FTL的蛋白表达下调,并呈剂量-效应关系;推测在矽肺纤维化进程中FTL可能具有负向调节效应。

Objective To observe the protein expression of ferritin light chain(FTL) in alveolar macrophages(AM) of patients with occupational silicosis(hereinafter referred to as silicosis). Methods The male patients with silicosis at stage Ⅰ, Ⅱ, and Ⅲ were separately selected as the silicosis groupⅠ, Ⅱ, and Ⅲ using judgment sampling method, with 15 patients in each group. Meanwhile, 15 male silicon dust workers with small lung shadows but not diagnosed as silicosis were selected as the control group. Bronchoalveolar lavage fluid was collected from the four groups, and AM was separated and purified, and protein was extracted after lysis of the AM. Western blotting was used to detect the relative expression of FTL protein in the AM. Results The relative expression of FTL protein in AM of silicosis groupⅠ, Ⅱ, and Ⅲ was lower than that in the control group(all P<0.05). The relative expression of FTL protein in AM decreased with the increase of silicosis stage(P<0.05).Conclusion The expression of FTL protein in AM was down-regulated in patients with silicosis in a dose-response manner. It is speculated that FTL may have a negative regulatory effect in the progress of silicosis fibrosis.