SLE患者血清外泌体环状RNA的差异表达分析

Study on the Differential Expression of Circular RNA in Serum Exosomes of Patients with SLE

目的采用测序技术分析系统性红斑狼疮(SLE)患者血清外泌体中差异表达的环状RNA(circRNA),为进一步探索SLE发病机制以及环状RNA作为SLE诊断生物标志物奠定基础。方法选取10例SLE患者(SLE组)及10例正常对照者(NC组)为研究对象,收集外周静脉血并分离血清,提取外泌体,然后提取外泌体中的circRNA,利用基因测序的方法筛选出差异性表达的circRNA。结果与正常对照相比,SLE患者血清外泌体circRNA种类减少,共有121个circRNA显著差异表达,包括54个上调和67个下调,其中大部分来自内含子基因区域。生物信息学分析显示Platelet activation信号通路是SLE发病机理中的重要信号途径。结论SLE患者血清外泌体中circRNA存在显著差异和特异性表达。显著差异表达的circRNA如chrY:13651007|13860076、chrY:13688616|13833086、chr17:22248380|22253301、chr10:39084961|39105726、chr2:233244474|233272478、chr17:39537965|39552828等可用作SLE发病机理研究的参考或补充。部分基因snoU13,SNORA31和SCARNA20可被视为SLE的潜在诊断生物标志物。

Objective Sequencing technology was used to analyze the differentially expressed circular RNAs(circRNAs)in serum exosomes of patients with systemic lupus erythematosus(SLE),laying a foundation for further exploration of the pathogenesis of SLE and the use of circular RNAs as biomarkers for SLE diagnosis.Methods 10 SLE patients(SLE group)and 10 normal controls(NC group)were selected as the research objects,peripheral venous blood was collected,serum was separated,exosomes were extracted,then circRNA in exosomes was extracted,and gene sequencing was used.Differentially expressed circRNAs were screened.Results Compared with normal controls,serum exosomal circRNA species in SLE patients were reduced,and a total of 121 circRNAs were significantly differentially expressed,including 54 up-regulated and 67 down-regulated,most of which were from intronic gene regions.Bioinformatics analysis showed that Platelet activation signaling pathway is an important signaling pathway in the pathogenesis of SLE.Conclusion There are significant differences and specific expression of circRNAs in serum exosomes of SLE patients compared with normal people.The significantly differentially expressed circRNAs such as chrY:13651007|13860076,chrY:13688616|13833086,chr17:22248380|22253301,chr10:39084961|39105726,chr2:233244474|233272478,chr17:39537965|39552828 can be used as references or supplements for the pathogenesis of SLE.The genes snoU13,SNORA31 and SCARNA20 can be considered as potential diagnostic biomarkers for SLE.