变应性鼻结膜炎模型小鼠的建立

Establishing a mouse model of allergic rhinoconjunctivitis

背景:研究过敏性结膜炎、过敏性鼻炎发病机制的相应动物模型已很成熟。而临床上过敏性鼻炎和过敏性结膜炎常同时存在,即过敏性鼻结膜炎,研究其发病机制的动物模型却鲜有报道。目的:为了深入探讨过敏性鼻结膜炎发病机制,建立一种小鼠变应性鼻结膜炎模型。方法:20只雌性C57小鼠(SPF级)随机分为2组:实验组于第0,4,7,14,21天行腹腔注射含卵清蛋白与佐剂Al(OH)_(3)的混悬液0.2 mL/只;间隔3 d后,每周连续5 d用5%卵清蛋白分别点每眼每鼻各10μL诱发过敏症状;对照组均用等量PBS替代,方法同实验组。在致敏和激发阶段,观察小鼠眼部、鼻部症状并进行评分;应用ELISA方法检测血清中卵清蛋白特异性IgE、总IgE及组胺水平;苏木精-伊红染色检测小鼠睑结膜及鼻黏膜组织变化。结果与结论:①实验组小鼠均诱发出鼻部、眼部过敏症状,鼻部出现过敏症状时间为(10.500±1.080)d,眼部出现过敏症状时间为(20.300±2.058)d;②实验组小鼠卵清蛋白特异性IgE、总IgE及组胺水平均高于对照组(P<0.05);③实验组小鼠睑结膜及鼻黏膜组织病理改变明显;④结果说明,实验成功建立了变应性鼻结膜炎小鼠模型。

BACKGROUND:Animal models for studying the pathogenesis of allergic conjunctivitis and allergic rhinitis have been well developed.Allergic rhinitis and allergic conjunctivitis often coexist in clinical practice,that is,allergic rhinoconjunctivitis.However,the animal model for studying its pathogenesis is rarely reported.OBJECTIVE:To establish a mouse model of allergic rhinoconjunctivitis for exploring its pathogenesis.METHODS:Twenty female C57 mice(SPF grade)were randomly divided into two groups:control group and experimental group.On days 0,4,7,14,and 21,each mouse in the experimental group was intraperitoneally given 0.2 mL of injectable suspension containing ovalbumin and adjuvant Al(OH)_(3).After an interval of 3 days,the eyes and nose of each mouse were given 5%ovalbumin,10μL per eye or per nose,5 continuous days per week to induce allergic symptoms.Mice in the control group were given the same amount of PBS.Ocular and nasal symptoms were observed and scored in the sensitization and provocation stages.The serum levels of ovalbumin-specific IgE,total IgE,and histamine were detected by ELISA method.Pathological changes of the palpebral conjunctiva and nasal mucosa in mice were observed using hematoxylin-eosin staining.RESULTS AND CONCLUSION:Allergic symptoms of the nose and eyes were induced in all the mice in the experimental group.The time from provocation until the emergence of allergic symptoms of the nose and eyes was(10.500±1.080)days and(20.300±2.058)days respectively.The levels of ovalbumin-specific IgE,total IgE,and histamine in the experimental group were significantly higher than those in the control group(P<0.05).There were remarkable pathological changes in the palpebral conjunctiva and nasal mucosa in the experimental group.To conclude,the mouse model of allergic rhinoconjunctivitis is successfully established.