状态性焦虑对小鼠睡眠-觉醒周期的影响

Effect of state anxiety on sleep-wake cycle in mice

目的在Triple test装置中,观察状态性焦虑对小鼠睡眠-觉醒周期的影响。方法选取18只ICR雄性小鼠,麻醉状态下,进行了皮层脑电电极和肌电电极的埋置,术后恢复7 d。先描记正常状态下连续24 h皮层脑电。每天首先利用三重复合装置(Triple test)复制小鼠状态性焦虑模型并做行为学评价,随后记录其24 h的皮层脑电,每天1次,连续7 d。结果行为学评价结果表明,与d 1相比,造模d 3、4、5、6在旷场(open field,OF)的中央格运动时间及中央格运动时间%明显降低(P<0.05,P<0.01),d 4-7在高架十字迷宫(EPM)中的开臂进入时间%及开臂进入次数%有明显增加(P<0.01),d 2、4在明暗穿梭箱(LDB)中的暗箱停留时间明显增加(P<0.05,P<0.01);脑电图(EEG)分析结果表明,与正常状态下的睡眠-觉醒周期相比,其睡眠和觉醒周期发生了明显的变化,其变化为在d 1、2、3、4、5和7清醒总时间和所占比例明显延长(P<0.05,P<0.01),NREM睡眠时间和所占比例明显缩短(P<0.05,P<0.01),在d 1、2、4 REM睡眠时间和所占比例明显增加(P<0.05)。结论利用Triple test装置复制的状态性焦虑小鼠伴有睡眠-觉醒周期的变化,且对日间睡眠的影响更加明显。

Aim To observe the effect of state anxiety on the sleep-wake cycle of mice in the Triple test.Methods Eighteen male ICR mice were selected,and the electroencephalogram(EEG)and electromyogram(EMG)electrodes were embedded in the cortex and neck muscles of the mice under the conditions of anesthesia.There was a continuous 24 h tracing of mice under normal conditions after the recovery period.The model replication and behavioral evaluation of the state anxiety took place in the Triple test and the mouse sleep for 24 h was recorded for 7 consecutive days after the daily test.Results The behavioral evaluation showed that compared with 1st d,the time spent in central and%time in OF on 3、4、5、7 d of modeling was significantly reduced(P<0.05;P<0.01);the number of entries and%time of 4-7 d in EPM signifi-cantly increased(P<0.01);2nd and 4th day of time spent in dark compartment of LDB increased significantly(P<0.05;P<0.01).The results of EEG analysis showed that compared with the sleep-wake cycle under normal conditions,the sleep and wake cycles had undergone significant changes,and the awakening time and its proportion were significantly prolonged(P<0.05;P<0.01),NREM sleep and its proportion markedly decreased on the 1-5 and 7 d(P<0.05;P<0.01),and a significant increase occurred in REM sleep and its proportion on 1st,2nd,and 4th day(P<0.05).Conclusion The sleep-wake cycle of state anxiety mice replicated with the Triple test changes,and the impact on daytime sleep is more obvious.