抵抗素样分子β在坏死性小肠结肠炎新生小鼠组织中的表达

Expression of resistin-like moleculeβin newborn mice with necrotizing enterocolitis

目的探讨抵抗素样分子β(RELMβ)在坏死性小肠结肠炎(NEC)新生小鼠组织中的表达。方法24只新生C57BL/6J小鼠随机分为2组,NEC组行人工喂养、缺氧、冷刺激、脂多糖灌胃(5 mg/kg)造模,而对照组行母乳喂养。ELISA检测血液中RELMβ水平,Western blot检测小肠组织中RELMβ表达。结果NEC组血液中RELMβ水平明显高于对照组(P<0.01),但小肠组织中RELMβ表达在两组间差异无统计学意义(P>0.05)。结论NEC新生小鼠血液中RELMβ水平升高。

Objective To investigate the expression of resistin-like moleculeβ(RELMβ)in newborn mice with necrotizing enterocolitis(NEC).Methods Twenty-four C57BL/6J newborn mice were randomized to two groups.NEC group were treated with artificially feeding,hypoxia,cold stimulation,and intragastric lipopolysaccharide(5 mg/kg),while control group with breast feeding.RELMβlevel in blood and small intestine was determined by ELISA and Western blot,respectively.Results Blood RELMβlevel was higher in NEC group than in control group(P<0.01),while RELMβexpression in small intestine was comparable between two groups(P>0.05).Conclusion RELMβoverexpression is present in blood of newborn mice with NEC.