泥炭地苔藓植物孢子生活力的快速检测

Rapid viability detection of peatland bryophyte spores

适量的烟气能够促进有性繁殖体萌发,但迄今尚无辅助烟气处理探究孢子生活力快速检测方法的研究报道。该文选择毛缘泥炭藓(Sphagnum fimbriatum)、中位泥炭藓(S.magellanicum)和粗叶泥炭藓(S.squarrosum)作为材料,分别使用亚甲基蓝染色法、四唑(TTC)染色法、碘-碘化钾(I_(2)-KI)染色法和红墨水染色法对泥炭藓孢子进行染色,并比照营养液、烟溶液+营养液培养的孢子萌发试验,对比研究泥炭地苔藓植物孢子生活力快速检测的最佳方法。结果表明:亚甲基蓝染色法的染色效果最为明显,TTC和I_(2)-KI均未能使泥炭藓孢子着色,孢子对红墨水虽有着色反应但不清晰;与营养液培养相比,添加烟溶液使毛缘泥炭藓、中位泥炭藓和粗叶泥炭藓孢子萌发率分别提高5%、5%和18%;使用亚甲基蓝染色的孢子染色率与经烟溶液处理过的孢子萌发率最为接近。综上认为,亚甲基蓝染色法能快速检测泥炭藓孢子的生活力。

Appropriate amount of smoke can promote germination of sexual propagules.However,rapid detection of spore viability combined with smoke treatment has not been reported up to now.In this paper,Sphagnum fimbriatum,S.magellanicum and S.squarrosum were selected as study species.Methylene blue,TTC,I_(2)-KI and red ink were used to dye spores,and nutrient solution and smoke+nutrient solution were used to culture spores,to test which is optimal method for rapid detection of peatland bryophyte spore viability.Of the four methods,methylene blue showed the most obvious dyeing effect while TTC and I_(2)-KI did not dye the Sphagnum spores and the spores had no clear and sharp reaction to red ink.Compared with those cultured with nutrient solution only,spores cultured with smoke+nutrient solution increased their germination by 5%,5%and 18%in S.fimbriatum,S.magellanicum and S.squarrosum,respectively.The frequency of Sphagnum spores dyed with methylene blue was the closest to that of spores germinated after smoke solution treatment.The results indicate that methylene blue dyeing is an ideal method to quickly detect viability of Sphagnum spores.