苹果梨果皮的7种酚类物质对链格孢的离体抑制作用

Inhibitory Effect of Seven Phenolic Compounds in Fruit Peel of Pingguoli Pear on Alternaria alternata in Vitro

为揭示苹果梨果皮的主要酚类物质对果实采后黑斑病菌链格孢(Alternaria alternata)生长的影响,在前期对发育及贮藏期梨果皮酚类物质含量分析的基础上,本文研究了苹果梨果皮的7种酚类物质处理对A. alternata孢子萌发、菌丝生长、菌丝干重及黑色素形成的影响。结果表明,除熊果苷外,酚酸和黄酮类物质均对A.alternata孢子萌发、菌丝生物量增加具有一定的抑制作用,且存在浓度依赖性,抑制效果依次为酚酸类>黄酮类>简单酚,其中阿魏酸的抑制作用较强,3 mg/mL的阿魏酸处理后A. alternata的孢子萌发、菌丝生长和菌丝干重分别较对照(0 mg/mL)组降低了98.5%、29.4%和22.2%。同时7种酚类物质处理后均不同程度的增加了A.alternata黑色素的含量。可见苹果梨果皮的酚类物质对A. alternata的生长和致病性具有重要的调控作用。

In order to reveal the effect of main phenolic substances on the growth of black spot Alternaria alternata after fruit harvesting, and on the basis of the analysis of the content of Pingguoli pear peel phenolic substances during the development and storage period, this paper studied the effects of seven phenolic substances in the Pingguoli pear peel on the spore germination, mycelium growth, mycelium dry weight and melanin formation. The results showed that, in addition to arbutin, phenolic acids and flavonoids had a certain inhibitory effect on the germination of A. alternata, and there was a concentration dependence, and the inhibitory effect was phenolic acids> flavonoids> simple phenols, in turn. The inhibitory effect of ferulic acid was strong, and the spore germination, mycelial growth and dry weight of mycelium treated with 3mg/mL ferulic acid were reduced by 98.5%, 29.4% and 22.2% respectively compared with the control(0 mg/mL). At the same time, the content of A. alternata melanin was increased in varying degrees after the treatment of seven phenolic substances. It can be seen that phenolic substances in Pingguoli pear peel have an important regulatory effect on the growth and pathogenicity of A. alternata.