不同种源蒙古栎遗传变异

Genetic Variation of Quercus mongolica from Different Provenances

分析蒙古栎EST序列和叶绿体全基因组SSR位点信息,开发SSR引物,对蒙古栎群体遗传变异进行分析,为蒙古栎分子标记辅助育种奠定基础。利用est-trimmer、CAP3对蒙古栎EST序列处理,通过perl结合MISA、Primer3和Electronic PCR查找SSR位点,设计引物并筛选验证。EST序列中发现SSR位点163个,主要重复类型为二核苷酸。叶绿体基因组上88个SSR位点以单核苷酸重复为主。7对引物共检测到37对等位基因,平均等位基因数(N_(a))=5.286,Shannon’s信息指数(I)=1.291,期望杂合度(H_(e))=0.622,多态性信息含量(P_(IC))=0.692;群体间遗传分化系数(F_(st))=0.147,基因流(N_(m))=1.933;AMOVA分析表明,种源间遗传变异占10.216%。蒙古栎种源群体间存在中等水平的遗传分化,其遗传变异主要来源于种源内,且种源间的遗传变异与地理距离无显著相关性。基于EST序列及叶绿体基因组SSR位点信息,有效开发SSR引物,为蒙古栎遗传多样性研究提供更多的标记支持。

We analyzed EST sequence and chloroplast genome-wide SSR loci,developed SSR primers and analyzed population genetic variation of Quercus mongolica.Est-trimmer and CAP3 were used to process EST sequence of Q.mongolica.SSR sites were found by Perl combined with MISA,Prime3 and electronic PCR,and primers were designed and screened for verification.The 163 SSR sites were found in EST sequence,and the main repeat types were dinucleotide.The 88 SSR loci in chloroplast genome were mainly single nucleotide repeats.The 37 alleles were detected by 7 primers,N_(a)=5.286,I=1.291,H_(e)=0.622,P_(IC)=0.692,F_(st)=0.147,N_(m)=1.933.AMOVA analysis showed that the genetic variation among provenances accounted for 10.216%.There is a medium level of genetic differentiation among provenances of Q.mongolica,and the genetic variation mainly comes from the provenance,and there is no significant correlation between the genetic variation and geographical distance.With EST sequence and the information of SSR loci in chloroplast genome,SSR primers were developed effectively,which would provide more marker support for the genetic diversity of Q.mongolica.