深海微生物来源细胞色素P450酶与天然产物生物合成

Deep-sea microbe derived cytochrome P450 enzymes and natural product biosynthesis

细胞色素P450酶广泛分布于自然界各种生命体内,具有底物结构类型丰富和催化反应类型多样的两大特点,被誉为自然界中的“万能催化剂”.P450酶可在温和条件下催化复杂有机化合物中惰性C—H键的区位或立体专一性高的氧化官能化,广泛参与天然产物的生物合成、异源物质的降解等生命活动,且在医药、化工、食品、农业等诸多领域具有广阔的应用价值和潜力.本文综述了参与深海来源天然产物生物合成的P450酶的底物结构特征及催化功能的研究现状,并对深海来源P450酶的发掘、功能鉴定及应用进行了展望.

Cytochrome P450 enzymes,recognized as"the most versatile catalyst"in nature,broadly exist in all kinds of life organisms and hold the greatest diversity of substrate structures and catalytic reaction types.P450 enzymes are capable of catalyzing the regio-and stereospecific oxidative functionalization of non-activated C—H bonds in complex organic compounds under mild conditions,and are widely involved in life activities,such as natural product biosynthesis and xenobiotic degradation.Accordingly,P450 enzymes show great application value and potential in medicine,chemical industry,food,agriculture,and many other fields.In this review,we summarize the research of status substrate structural characteristics and catalytic functions of P450 enzymes involved in biosynthesis of deep-sea natural products and provided prospects for the discovery,functional identification and application of deep-sea derived P450 enzymes.