摘要
目的:基于指纹图谱和网络药理学分析白术中潜在质量标志物(Q-Marker)并测定其含量。方法:采用Waters Sun Fire C_(18)色谱柱(250 mm×4.6 mm,5μm),以水(A)-乙腈(B)为流动相,梯度洗脱,流速1 m L·min^(-1),检测波长切换测定,柱温30℃,进样量10μL,建立白术药材指纹图谱,对32批白术药材进行相似度评价,确认共有峰并进行指认,再通过网络药理学方法构建“活性成分-靶点-通路”网络图,预测Q-Marker,并测定其含量。结果:建立了32批白术药材指纹图谱,确认了28个共有峰,通过白术对照品指认4个色谱峰,分别为白术内酯Ⅰ、白术内酯Ⅱ、白术内酯Ⅲ、苍术酮;经网络药理学确认以上4种成分为活性成分,可作用于16个核心靶点、20条关键通路发挥抗癌、抗炎、改善胃肠道疾病作用,初步预测白术内酯Ⅰ、白术内酯Ⅱ、白术内酯Ⅲ、苍术酮为潜在Q-Marker,白术药材中其总质量分数不低于1.79 mg·g^(-1)。结论:白术潜在Q-Marker预测分析为建立一整套质量控制评价体系提供参考,为阐明其药效物质基础的作用机制奠定基础。
Objective:To analyze the potential quality marker(Q-Marker)in Atractylodis Macrocephalae Rhizoma and determine its content based on fingerprints and network pharmacology.Methods:Waters Sun Fire C_(18) chromatographic column(250 mm×4.6 mm,5μm)was used with water(A)-acetonitrile(B)as mobile phase,gradient elution,flow rate of 1 m L·min^(-1),detection wavelength switching measurement,column temperature of 30℃ and injection volume of 10μL to establish the fingerprints of Atractylodis Macrocephalae Rhizoma.of 32 batches of Atractylodis Macrocephalae Rhizoma were evaluated,the common peaks were confirmed and identified,and the“active ingredient-target-pathway”network was subsequently constructed with network pharmacological method to predict Q-Marker and determine its content.Results:The fingerprints of 32batches of Atractylodis Macrocephalae Rhizoma were established,and 28 common peaks were confirmed.Four chromatographic peaks were identified by comparing the reference substances of Atractylodis Macrocephalae Rhizoma,which were Atractylenlolide Ⅰ,Atractylenolide Ⅱ,Atractylenolide Ⅲ and Atractylone,respectively;It is also confirmed by network pharmacology that the above four components are active components,that can act on 16 core targets and 20 key pathways to play the roles of anti-cancer,anti-inflammatory and improving gastrointestinal conditions.It is preliminarily predicted that Atractylenolide Ⅰ,Atractylenolide Ⅱ,Atractylenolide Ⅲ and Atractylolone are potential Q-Markers,and the total mass fraction of Atractylodis Macrocephalae Rhizoma is not less than 1.79 mg·g^(-1).Conclusion:The prediction and analysis to potential Q-Markers of Atractylodis Macrocephalae Rhizoma will provide references for the establishment of a set of quality control evaluation system and will lay a foundation for clarifying the material basis and mechanism of its efficacy.
作者
李晴
朱香梅
石雨荷
朱珏
王智
刘湘丹
周日宝
童巧珍
Li Qing;Zhu Xiangmei;Shi Yuhe;Zhu Jue;Wang Zhi;Liu Xiangdan;Zhou Ribao;Tong Qiaozhen(School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China;Key Research Laboratory of Germplasm Resources and Standardized Planting of Genuine Regional Medicinal Materials Produced in Hunan Province,Changsha 410208,China;Key Laboratory of Modem Research on TCM,Colleges and Universities of Hunan Province,Changsha 410208,China)
出处
《中国药事》
CAS
2022年第4期404-416,共13页
Chinese Pharmaceutical Affairs
基金
湖南省科技厅中央引导地方科技发展专项资金项目(编号2019XF5069)
湖南省科技厅科技特派员服务乡村振兴项目(编号2021NK4240)
湘产大宗道地药材平术高品质生态种植及精准扶贫示范研究(编号19A367)。
关键词
白术
质量标志物
指纹图谱
网络药理学
白术内酯Ⅰ
白术内酯Ⅱ
白术内酯Ⅲ
苍术酮
Atractylodis Macrocephalae Rhizoma
quality markers
fingerprints
network pharmacology
Atractylenolide Ⅰ
Atractylenolide Ⅱ
Atractylenolide Ⅲ
Atractylolone