摘要
Objective:The objective of this study was to explore the mechanism of Guan Gefang(GGF);raw rhubarb 30 g,cassia arboreal 30 g,raw oyster 30 g,ground elm 60 g,and dandelion 30 g.kidney protection.Materials and Methods:Thirty-six Sprague Dawley rats were randomly divided into a control group(Group N),a sepsis control group(Group S),and a sepsis+GGF group(Group G).For Group N,8 ml/kg 0.9%NaCl was used as an enema;for Group S,cecal ligation and puncture(CLP)method was used for modeling and 8 ml/kg 0.9%NaCl was used as an enema;and Group G,CLP was used for modeling and 8 ml/kg GGF was used as an enema.All of the enemas were applied once daily for 4 days.The indices of serum creatinine(SCr),blood urea nitrogen(BUN),uric acid(UA),mammalian target of rapamycin(mTOR),Janus kinase 2(JAK2)were compared across each group.Results:Compared to Group S,Group G had lower levels of SCr,BUN,and UA(P<0.05),while the activities of mTOR and JAK2 were significantly inhibited.Conclusion:GGF may have inhibited the JAK2 or mTOR signaling pathways to protect the rats’kidneys,which had sepsis-associated acute kidney injury.
