利用双重数字PCR方法检测进口粮谷中携带地肤的EPSPS基因拷贝数

Application of Duplex Droplet Digital PCR for Detection of EPSPS Gene Copy Number of Kochia scoparia in Imported Grains

建立了双重数字PCR检测地肤EPSPS基因拷贝数的方法,对进口粮谷中携带的8个地肤样品进行检测,发现2个样品的EPSPS相对拷贝数小于1,6个样品EPSPS相对拷贝数大于5,其中一个样品EPSPS相对拷贝数高达45.67。由此可见,进口北美粮谷携带抗草甘膦地肤的比例很高。

A double digital PCR method was established to detect the EPSPS gene copy number of Kochia scoparia. Eight samples of Kochia scoparia carried in imported grains were detected.It was found that the relative copy number of EPSPS of 2 samples was less than 1, the relative copy number of EPSPS of 6 samples was greater than 5, and the relative copy number of EPSPS of one sample was as high as 45.67.It can be seen that the proportion of imported North American grains carrying glyphosate-resistant kochia is very high.