以玉米秸秆为基质的微生物发酵产多糖的工艺优化

Process Optimization of Polysaccharide Production by Microbial Fermentation with Corn Stalks as Substrate

以玉米秸秆为底物,通过培养3株放线菌组成的7^(#)菌群来发酵生产多糖。采用乙醇沉淀法对玉米秸秆发酵后的水溶性多糖进行了分离,并利用Minitab 15对7^(#)菌群降解玉米秸秆的发酵工艺进行优化设计和分析。结果表明,7^(#)菌群培养后多糖含量增加了677.54 mg/L,并随培养时间呈上升趋势。培养时间和秸秆量对多糖含量有显著影响,培养时间12 d、秸秆量50.4 g/L时多糖含量的最佳值为2 146.90 mg/L。

Corn stalks was used as a substrate to produce polysaccharides by culturing 7^(#)flora composed of 3 actinomycetes. The ethanol precipitation method was used to separate the water-soluble polysaccharides after fermentation of corn stalks, and the fermentation process of 7^(#)bacteria degrading corn stalks was optimized and analyzed by Minitab15.The results showed that the polysaccharide content of 7^(#)bacteria group increased by 677.54 mg/L after culturing, and showed an upward trend with the culturing time.Cultivation time and straw amount had a significant effect on polysaccharide content. The optimum value of polysaccharide content was 2 146.90 mg/L when the cultivation time was 12 d and the straw amount was 50.4 g/L.