摘要
为了鉴别和研究迷迭香生长习性及其精油组成特点,以生长在河南信阳的两种迷迭香绿色窄叶、宽叶为研究对象,考察其生物学性状,并利用气相-质谱仪(GC-MS)分析了精油的化学成分;以清除羟基自由基(·OH)能力为指标,考察当地迷迭香精油的抗氧化活性。结果表明,当地窄叶迷迭香精油主要成分包括桉叶素(29.826%)、(1S)-1,7,7-三甲基-双环[2.2.1]庚-2-酮(18.939%)、γ-松油烯(8.051%)、冰片(7.434%)、莰烯(5.542%);宽叶迷迭香精油主要成分有(1R)-2,6,6-三甲基二环[3.1.1]庚-2-烯(28.141%)、桉叶素(23.076%)、(1S)-4,6,6-三甲基二环[3.1.1]庚-3-烯-2-酮(9.758%)、冰片(6.244%)、莰烯(4.833%)等。此两种迷迭香精油在1~1.8 mg/mL浓度范围内,羟基自由基清除率变化呈线性上升趋势。当精油浓度为1.8 mg/mL时,窄叶迷迭香精油对羟基自由基清除率达到90.33%,宽叶迷迭香精油羟基自由基清除率达到87.06%。窄叶、宽叶迷迭香品种不同,精油含量及成分有差别,窄叶精油对羟基自由基清除能力比相同浓度下宽叶精油的清除能力强,精油的清除能力均好于维生素C(VC)。
To identify and obtain the growth habit and essential oil composition of rosemary officinalis,the biological characters of two kinds of green leaf between narrow and wide rosemary officinalis grown in Xinyang,Henan Province were investigated.The chemical components of the essential oil were analyzed by gas chromatography-mass spectrometry(GC-MS).The antioxidative activity of rosemary officinalis essential oil was investigated with the ability of scavenging hydroxyl free radical(·OH)as an index.The main components of essential oil from local narrow rosemary officinalis include eucalyptus(29.826%),(1S)-1,7,7-three methyl-bicyclic[2.2.1]geng-2-ketone(18.939%),γ-terpinene(8.051%),borneol(7.434%),camphene(5.542%).The main components of rosmarinus officinalis essential oil were:(1R)-2,6,6-three methyl bicyclic[3.1.1]heptane-2-(28.141%),eucalyptus(23.076%),(1S)-4,6,6-three methyl bicyclic[3.1.1]heptane-3-ene-2-ketone(9.758%),borneol(6.244%),and borneol(4.833%).The scavenging rate of hydroxyl radical in the two kinds of rosemary officinalis essential oils increased linearly in the concentration range of 1~1.8 mg/mL.When the concentration of essential oil was 1.8 mg/mL,the scavenging rate of narrow rosemary officinalis essential oil to hydroxyl radical reached 90.33%,and the scavenging rate of hydroxyl radical of rosmarinus officinalis essential oil reached 87.06%.The content and composition of essential oil were different in different varieties of narrow and wide leaf rosemary officinalis.The scavenging ability of narrow leaf essential oil was stronger than that of wide leaf essential oil at the same concentration,and the scavenging ability of essential oil was better than vitamin C(VC).
作者
叶兆伟
李金平
叶润
李琪琪
陈琼
YE Zhao-wei;LI Jin-ping;YE Run;LI Qi-qi;CHEN Qiong(School of Biology and Pharmaceutical Engineering,Xinyang Agriculture and Forestry University,Xinyang 464000,China;Henan Xibanxia Co.,Ltd.,Xinyang 464000,China)
出处
《化学试剂》
CAS
北大核心
2022年第5期662-667,共6页
Chemical Reagents
基金
河南省重大科技专项项目(201111310900)
信阳市创新应用专项项目(20190022)
校级科技创新团队建设项目(CXTD-201805)。
关键词
迷迭香
精油
GC-MS
羟基自由基
抗氧化能力
rosemary officinalis
essential oil
GC-MS
hydroxyl radical
antioxidant ability