一种BAPTA荧光探针的合成、光谱性能与识别机制

Synthesis,Spectral Properties and Recognition Mechanism of A BAPTA Fluorescent Probe

以苯乙烯类发色团苯并咪唑作为荧光基团、多羧酸受体作为识别基团,基于分子内电荷转移机制定向构筑探针结构,合成得到一种对Zn^(2+)、Cu^(2+)、Hg^(2+)具有响应的荧光探针。定性及定量识别实验显示,探针对目标物Zn^(2+)、Cu^(2+)、Hg^(2+)表现特征响应,探针与目标物1∶1定量结合,荧光性能随pH、时间变化而相对稳定、线性关系良好,能够用于μmol/L浓度范围目标离子的定性检测和定量分析。此外,通过荧光、紫外等光谱学手段与核磁分析,初步阐明了探针对Cu^(2+)、Hg^(2+)和Zn^(2+)的不同识别机制,对于探针结构优化与性能筛选研究提供了依据。

By incorporating polycarboxylic receptor and benzimidazole as the fluorophore,based on the recognition mechanism of intramolecular charge transfer,a fluorescent probe for the detection of aqueous Zn^(2+)/Cu^(2+)/Hg^(2+)was synthesized.Qualitative and quantitative analyses indicated the probe-Zn^(2+)/Cu^(2+)/Hg^(2+)systems gave off characteristic fluorescence responses with a 1∶1 complexation stoichiometry,demonstrating steady optical properties versus pH/time and good linear relationships,which could be applied to qualitative and quantitative detections of target ions at the concentration ofμmol/L.Furthermore,fluorescence,UV-Vis and;HNMR were adopted to explore the different combination mechanisms in the probe-Zn^(2+)/Cu^(2+)/Hg^(2+)systems,offering the evidence for the research concerning the probe′s structural modification and properties optimization.