室温下柱层析法同时提取刺玫果肉中维生素C和花色苷及其体外活性研究

Simultaneous Extraction of Vitamin C and Anthocyanins from the Fruit Pulp of Rosa davurica Pall.by Column Chromatography at Room Temperature and Their Activities in Vitro

探究柱层析法提取刺玫果肉中维生素C和花色苷的工艺条件及体外活性。以提取液中维生素C质量和花色苷吸光度为指标,通过对溶剂浓度、pH、酸度调节剂、吸涨率(MV)、浸泡平衡时间等因素的考察确定最佳提取工艺。通过测定刺玫果肉维生素C、花色苷对α-葡萄糖苷酶的抑制率及对1,1-二苯基-2-三硝基苯肼(DPPH·)的清除率,考察其体外降血糖、抗氧化活性。结果表明,在最佳提取条件下,刺玫果肉维生素C和花色苷的提取率均在95%以上,浸膏中维生素C的含量达到15%以上、花色苷的色价为8.07。刺玫果肉维生素C、花色苷提取物抑制α-葡萄糖苷酶的活性均强于阳性对照阿卡波糖;其清除DPPH·的IC;值分别为0.0031、0.0221 mg/mL。柱层析法可以同时提取刺玫果肉中的维生素C和花色苷,避免因单独地提取一种活性成分而造成原料和溶剂的浪费,研究结果为刺玫果开发提供了科学依据。

The extraction conditions and activities in vitro of vitamin C and anthocyanins from the fruit pulp of Rosa davurica Pall.by column chromatography were investigated.Using quality of vitamin C and absorbance of anthocyanin as indexes,the optimal extraction process was determined base on the examination of factors such as solvent concentration,pH,acidity regulator,swelling-up rate and immersion equilibrium time.Both theα-glucosidase inhibitory rate and the DPPH·clearance rate of vitamin C and anthocyanin were determined to investigate their hypoglycemic and antioxidant activities in vitro.Under the optimal extraction conditions,the extraction rates of vitamin C and anthocyanin in the fruit pulp of Rosa davurica Pall.were above 95%,the content of vitamin C in extract was above 15%,and the color value of anthocyanin was 8.07.Theα-glucosidase inhibition activity of vitamin C and anthocyanin in extract of the fruit pulp of Rosa davurica Pall.was stronger than positive control acarbose.The IC;of DPPH·were 0.0031,0.0221 mg/mL,respectively.Column chromatography can simultaneously extract vitamin C and anthocyanins which may avoid the waste of raw materials and solvents caused by extracting one active ingredient at one time.The results provided scientific basis for the R&D of Rosa davurica Pall.