生活饮用水中总大肠菌群三种不同检测方法比较

Comparison of Three Different Detection Methods for Total Coliform in Drinking Water

本文采用传统多管发酵法、滤膜法与固定酶底物法检测生活饮用水中总大肠菌群数量,比较了3种检测方法的差异性。结果表明,酶底物法检测标准菌株在真实值范围的波动偏差最小。其中,多管法具有成本低、人工成本高的特点;滤膜法具有检测时长相对较短且适用于杂质较少的水样;酶底物法操作简便快速,对检测环境的要求最低,准确度最高,不易造成环境的二次污染。

In this paper,the traditional multi-tube fermentation method,membrane filtration method and fixed enzyme substrate method were used to detect the total coliform in drinking water,and the differences of the three methods were compared.The results showed that the fluctuation deviation of the standard strain detected by enzyme substrate method was the smallest in the true value range.Among them,multi-pipe method has the characteristics of low cost and high labor cost.The membrane method has a relatively short detection time and is suitable for water samples with less impurities.Enzyme substrate method is simple and fast to operate,has the lowest requirements for the detection environment,the highest accuracy,and is not easy to cause secondary pollution of the environment.