急性脑梗死患者外周血中LncRNA RP11-147L13.8的表达水平及临床意义

Expression level and clinical significance of LncRNA RP11-147L13.8 in peripheral blood of patients with acute cerebral infarction

目的探讨急性脑梗死患者外周血中LncRNA RP11-147L13.8的表达水平及临床意义。方法随机选择在我科住院的50例急性脑梗死患者(病例组)和50例非脑梗死患者(对照组),采用实时荧光定量PCR检测外周血中LncRNA RP11-147L13.8在病例组和对照组中的表达水平,同时探讨其与脑梗死的相关性。结果脑梗死组患者外周血中LncRNA RP11-147L13.8的表达水平明显高于对照组(P<0.05),外周血LncRNA RP11-147L13.8的表达水平与急性脑梗死的发生呈正相关,并且LncRNA RP11-147L13.8是急性脑梗死发生的独立危险因素。LncRNA RP11-147L13.8诊断急性脑梗死的曲线下面积是0.750,临界值为0.40,敏感度为0.66,特异度为0.74。结论LncRNA RP11-147L13.8在脑梗死中会差异表达,有望成为诊断急性脑梗死的一种潜在生物标记物,同时为临床诊疗提供新的思路。

Objective To investigate the expression level and clinical significance of LncRNA RP11-147L13.8 in peripheral blood of patients with acute cerebral infarction.Methods Fifty patients with acute cerebral infarction(case group)and 50 patients without cerebral infarction(control group)hospitalized in our department were randomly selected,and the expression level of LncRNA RP11-147L13.8 in peripheral blood was detected by real-time fluorescence quantitative PCR in case group and control group,and the correlation between LncRNA RP11-147L13.8 and cerebral infarction was investigated.Results The expression level of LncRNA RP11-147L13.8 in peripheral blood of patients with cerebral infarction was significantly higher than that of control group(P<0.05).The expression level of LncRNA RP11-147L13.8 in peripheral blood was positively correlated with the occurrence of acute cerebral infarction,and LncRNA RP11-147L13.8 was an independent risk factor for the occurrence of acute cerebral infarction.LncRNA RP11-147L13.8 in the diagnosis of acute cerebral infarction had an area under the curve of 0.750,a critical value of 0.40,a sensitivity of 0.66 and a specificity of 0.74.Conclusion LncRNA RP11-147L13.8 is differentially expressed in cerebral infarction,which is expected to become a potential biomarker for the diagnosis of acute cerebral infarction and provide new ideas for clinical diagnosis and treatment.