首次从人创面分泌物中分离出创口鲍特菌及病例简报

Bordetella trematum isolated from human wound secretion:first case report

对1株从人感染创面分泌物分离的特殊菌进行鉴定和药敏试验。收集该患者的创面分泌物标本,采用血琼脂平板和麦康凯琼脂平板进行分离培养。VITEK 2 Compact全自动微生物鉴定系统鉴定为鲍特菌属(置信度为97%),VITEK MS全自动快速微生物质谱检测系统和16S rRNA分析鉴定结果均为创口鲍特菌(置信度均为99.9%)。药敏试验结果显示,创口鲍特菌对头孢唑林、头孢他啶、头孢吡肟、头孢曲松、庆大霉素、妥布霉素、左氧氟沙星、复方磺胺甲口恶唑均耐药,对哌拉西林/他唑巴坦、亚胺培南和美罗培南均为中介,仅对阿米卡星敏感。此病例为国内首次从临床标本中分离出创口鲍特菌的报告,为指导创口鲍特菌感染的快速、准确鉴定和临床合理用药提供参考。

One bacterial strain isolated from human infection wound secretion was identified and performed antimicrobial susceptibility testing.Wound secretion specimen of the patient was collected,blood agar plate and McConkey agar plate were used for isolation and culture.Bacterial strain was identified as Bordetella spp.by VITEK 2 Compact automatic microbial identification system(confidence 97%),Bordetella trematum(B.trematum)was identified by VITEK MS automatic rapid microbial mass spectrometry detection system and 16S rRNA analysis(confidence 99.9%).Antimicrobial susceptibility testing showed that B.trematum was resistant to cefazolin,ceftazidime,cefepime,cefatriaxone,gentamicin,tobramycin,levofloxacin,and compound sulfamethoxazole,it is mediate resistant to piperacillin/tazobactam,imipenem and meropenem,while susceptible to amikacin.This case is the first report on the isolation of B.trematum from clinical specimens in China,which provides a reference for guiding the rapid and accurate identification of B.trematum infection and clinical rational antimicrobial use.