miRNA-126及runt相关转录因子3在支气管哮喘患儿外周血中的表达及意义

Expression and significance analysis of miRNA-126 and runt-associated transcription factor 3 in peripheral blood of children with bronchial asthma

目的探讨miRNA-126与runt相关转录因子3(RUNX3)在支气管哮喘患儿中的表达及意义。方法选取河南省儿童医院收治的80例支气管哮喘患儿为研究对象,根据疾病的临床进展分为急性期组(n=42)与缓解期组(n=38);同期选取健康体检的儿童40例为对照组。qRT-PCR法检测3组受试儿外周血中miRNA-126水平,Western blot检测RUNX3蛋白表达水平;分析哮喘组患儿miRNA-126与RUNX3水平的相关性;ROC曲线分析miRNA-126及RUNX3在哮喘中的诊断价值。结果与对照组相比,缓解期组与急性期组的miRNA-126表达水平均显著增加(P<0.05),RUNX3蛋白水平显著降低(P<0.05);且急性期组miRNA-126水平显著高于缓解期组,RUNX3水平显著低于缓解期组(P<0.05);miRNA-126与RUNX3水平呈显著负相关(P<0.05);miRNA-126与RUNX3诊断哮喘的AUC分别为0.933,0.847,诊断哮喘临床分期的AUC分别为0.696,0.798。结论miRNA-126与RUNX3可能协同参与儿童支气管哮喘的发生发展,可作为哮喘诊断及病情评估的潜在辅助指标。

Objective To investigate the expression and significance of miRNA-126 and RUNX3 in children with bronchial asthma.Methods Eighty children with bronchial asthma treated in Henan Children's Hospital were selected as research subjects,and they were divided into acute stage group(n=42)and remission stage group(n=38)according to the clinical progress of the disease.At the same time,40 healthy children were selected as the control group.The levels of miRNA-126 in peripheral blood of the three groups were detected by qRT-PCR,and the expression level of RUNX3 protein was detected by Western blot;The correlation between miRNA-126 and RUNX3 level in asthmatic groups was analyzed.The diagnostic value of miRNA-126 and RUNX3 in asthma was analyzed by ROC curve.Results Compared with the control group,the expression level of miRNA-126 was significantly increased(P<0.05),while RUNX3 protein was significantly decreased(P<0.05)in both the remission and acute stage groups.The level of miRNA-126 in acute stage group was significantly higher than that in remission group,while the level of RUNX3 protein was significantly lower than that in remission group(P<0.05).The miRNA-126 was significantly negatively correlated with RUNX3 level(P<0.05).The AUCs of miRNA-126 and RUNX3 in diagnosing asthma were respectively 0.933 and 0.847,and the AUCs for the diagnosis of clinical stages of asthma were respectively 0.696 and 0.798.Conclusion The miRNA-126 and RUNX3 may be synergistically involved in the development of bronchial asthma in children,which may serve as a potential auxiliary indicator for the diagnosis and evaluation of asthma.