早熟大豆种质资源萌发期耐旱性评价

Evaluation of Drought Tolerance During Germination of Early-maturing Soybean Germplasm Resources

为筛选黑龙江省北部早熟耐旱大豆种质资源,本研究采用15%PEG-6000对113份早熟大豆种质进行萌发期模拟干旱胁迫处理,测定干旱处理下大豆萌发期的相关指标。结果表明:干旱胁迫对大豆种子萌发指标影响程度依次为发芽势>发芽指数>发芽率>芽长;采用隶属函数法将供试材料分成5个等级,1级耐旱型种质6份,2级较耐旱型种质73份,3级中间型种质31份,4级干旱较敏感型种质1份,5级干旱敏感型种质2份。通过萌发相对性状的各隶属函数值进行系统聚类分析,在欧式距离4.0时,可将参试种质分成5类,第Ⅰ类(耐旱型)1份;第Ⅱ类(较耐旱型)1份;第Ⅲ类(中间型)36份;第Ⅳ类(干旱较敏感型)73份;第Ⅴ类(干旱敏感型)2份。综合两种评价得出耐旱种质1份,为选11(黑河18×冀豆17)×黑河18。

In order to screen early-maturing drought-tolerant soybean germplasm resources in northern Heilongjiang Province,113 early-maturing soybean germplasm were treated with 15%PEG-6000 to simulate drought stress during the germination period in this study,and the relevant indexes of germination period were measured.The results showed that drought stress affected soybean seeds in the following order:germination potential>germination index>germination rate>shoot length.The results were evaluated by the affiliation function method,and the test materials were classified into five classes:6 drought-tolerant germplasms of class 1,73 more drought-tolerant germplasms of class 2,31 intermediate germplasm of class 3,1 more drought-sensitive germplasm of class 4,and 2 drought-sensitive germplasms of class 5.The germplasm was classified into five classes at a Euclidean distance of 4.0,with one class I(drought-tolerant),two classes II(more drought-tolerant),36 classes III(intermediate),73 classes IV(more drought-sensitive),and two classes V(drought-sensitive),by systematic cluster analysis of each affiliation function value of sprouting relative traits.The two evaluations resulted in one drought-tolerant germplasm of selection 11(Heihe 18×Jidou 17)×Heihe 18.The selected drought-tolerant germplasm will provide superior germplasm resources for drought-resistant breeding of early-maturing soybean in northern Heilongjiang Province.