NaCl胁迫对不同谷子品种萌发的影响

Effect of NaCl Stress on Different Millet Varieties Germination

为了探究NaCl胁迫对不同谷子品种萌发的影响,采用纸间发芽法对12份谷子品种进行150 mmol·L^(-1) NaCl溶液处理,对照组采用蒸馏水处理。对谷子种子萌发时的9个性状指标进行测定并计算其相对值,采用相关性、聚类和主成分分析法对其相对值进行综合评价,并筛选耐盐谷子品种。结果表明:在NaCl胁迫下,12份谷子品种在萌发期根干重、鲜重和长度相对值的变异系数分别大于芽干重、鲜重和长度相对值;指标间存在极显著正相关性,体现在相对种子发芽势与相对发芽率、相对发芽指数之间,以及相对发芽率与相对发芽指数之间;发芽势、发芽率、发芽指数、根鲜重、芽鲜重、芽长和根长共7个指标的相对值与3个主成分因子正载荷较大,可以作为筛选谷子耐盐的关键指标;聚类分析后12份谷子品种被划分为4类,综合隶属函数得分进行排序获得3份耐盐谷子品种,分别为磨里谷、晋谷29和红谷09-1。

In order to investigate the effect of NaCl stress on germination of different millet varieties and to screen salt-tolerant millet varieties,12 millet varieties were treated with 150 mmol·L^(-1) NaCl solution using the inter paper germination method and the control group was treated with distilled water.Nine trait indicators of millet seed germination were measured and their relative values were calculated,and correlation,clustering and principal component analysis were used to analyze their relative values and evaluate them comprehensively.The results showed that the coefficients of variation of the relative values of dry weight,fresh weight and length of roots were greater than those of shoots,respectively,in 12 millet varieties during germination under NaCl stress.There were highly significant positive correlations among the indicators,which were reflected between the relative values of seed germination potential and two indicators of germination rate and germination index,and between the relative values of germination rate and germination index.The relative values of germination potential,the relative values of seven indicators,including germination potential,germination index,root fresh weight,shoot fresh weight,shoot length and root length,were positively loaded with the three principal component factors,which can be used as key indicators for screening millet salt tolerance.After cluster analysis,12 millet varieties were divided into four categories,and three salt-tolerant millet varieties were obtained by ranking the integrated affiliation function scores,which were Moligu,Jingu 29 and Honggu 09-1,respectively.