Box-Behnken设计-响应面法优化菜头肾酚类成分的提取工艺

Optimization of Extraction Process for Polyphenols of Strobilanthes sarcorrhizus C.Ling by Box-Behnken and Response Surface Methodology

为促进菜头肾中酚类成分的开发利用,本研究对乙醇浓度、料液比和温度进行单因素研究,应用Box-Behnken响应面分析法建立模型,以多酚提取量作为响应值,确定菜头肾中多酚提取的最佳条件。结果表明:通过响应面法预测的回归模型分析,确定了菜头肾多酚的最佳工艺条件为提取温度50℃,料液比1∶22,乙醇浓度75%,此工艺条件下多酚得率为5.896 mg·g^(-1),预测值与实际值一致,表明该工艺条件可提高菜头肾多酚的得率。

In order to promote the development and utilization of Strobilanthes sarcorrhizus C.Ling,this study conducted a single factor study on ethanol concentration,solid-liquid ratio and temperature.Box-behnken response surface analysis was used to establish a model,and the extraction amount of polyphenols was used as the response value to determine the optimal conditions for the extraction of polyphenols from Strobilanthes sarcorrhizus C.Ling.The results showed that the optimal extraction conditions were determined as extraction temperature of 50℃,solid-liquid ratio of 1∶22 and ethanol concentration of 75%.The yield of polyphenols was 5.896 mg·g^(-1)under these conditions.The predicted value was consistent with the actual value,which indicated that the process could improve the yield of polyphenols.