羊栖菜醇提物中岩藻黄素的检测

Determination of Fucoxanthin in Alcohol Extract of Sargasum Fusiforme

建立了羊栖菜醇提取物中岩藻黄素的薄层色谱和液相色谱双重定性及定量方法。采用薄层色谱将样品与岩藻黄素标准品和玉米黄素标准品进行层析对照,再经C_(18)-HPLC-PDA进行分离。以乙腈-水(体积比4∶1)为流动相,柱温25℃,流速0.8 mL/min,进样量10μL,在波长450 nm下检测,外标法定量。结果显示,试样色谱与岩藻黄素标准品相应的位置上显示相同颜色的斑点,且试样和岩藻黄素标准品比移值相差范围在3%以内,与玉米黄素标准品相应的位置上无斑点。经HPLC分离,岩藻黄素在0~10.0 mg/L范围内线性关系良好,相关系数为0.99995,回收率超过97.5%,相对标准偏差(RSD)为0.7%~1.6%。此方法准确、重复性好,可为羊栖菜醇提物中岩藻黄素定性定量提供依据。

A dual qualitative and quantitative method for fucoxanthin in the alcohol extract of brown seaweed by TLC and HPLC was established.The sample was compared with the fucoxanthin standard and zeaxanthin standard by TLC,and then separated by C_(18)-HPLC-PDA.Acetonitrile-water(volume ratio 4∶1)was used as the mobile phase,the column temperature was 25℃,the flow rate was 0.8mL/min,and the injection volume was 10μL.The samples were detected at the wavelength of 450 nm and quantified by external standard method.The results demonstrated that the chromatogram of the samples showed the same color spots on the corresponding position of the fucoxanthin standard,and the difference of the specific shift value between the sample and fucoxanthin standard was within 3%,and there were no spots on the corresponding position of zeaxanthin standard.After separated by HPLC,the linear relationship of fucoxanthin was good in the range of 0~10.0mg/L.The correlation coefficient was 0.99995,the recovery rate was over 97.5%,and the relative standard deviation(RSD)was 0.7%~1.6%.This method is accurate and with good repeatability,which can provide a basis for the qualitative and quantitative determination of fucoxanthin in alcohol extract of Sargasum Fusiforme.