黄芪多糖对肝癌Hep G2.215细胞的抑制作用及其机制研究

Study on the inhibitory effect of Astragalus polysaccharides on Hep G2.215 cells and its mechanism

目的探讨黄芪多糖对肝癌Hep G2.215细胞体外增殖的抑制作用及其作用机制。方法CCK-8法测定不同浓度黄芪多糖作用于Hep G2.215细胞48 h、72 h后对其增殖影响;平板细胞克隆实验测定不同浓度黄芪多糖对Hep G2.215细胞克隆生长的影响;流式细胞术检测黄芪多糖对Hep G2.215细胞周期和凋亡的影响。结果CCK-8检测结果显示,当黄芪多糖给药浓度达1000 μg/ml时,对Hep G2.215细胞生长具有抑制作用,且与对照组相比差异有统计学意义(P<0.05)。平板细胞克隆实验表明,低浓度(100 μg/ml)的黄芪多糖对Hep G2.215细胞克隆形成具有抑制作用,与对照组比较,差异有统计学意义(P<0.05)。流式细胞术检测结果显示,G2/M期细胞比例与对照组相比呈下降趋势(P<0.05),经过黄芪多糖处理的Hep G2.215细胞,细胞凋亡率高于对照组(P<0.05)。结论黄芪多糖对肝癌Hep G2.215细胞生长具有抑制作用,其机制可能是通过阻滞Hep G2.215细胞进入G2/M期,激活了细胞凋亡系统,从而诱导细胞凋亡。

Objective Investigate the inhibitory effects of Astragalus polysaccharides on the proliferation of HBV positive hepatocellular carcinoma cells Hep G2.215 in vitro and the mechanism of this process. Methods CCK-8 assay was used to determine the effect of different concentrations of Astragalus polysaccharides on the proliferation of Hep G2.215 cells for 48 h and 72 h. The effects of different concentrations of Astragalus polysaccharides on the clonal growth of Hep G2.2.15 cells were determined by plate cell cloning assay. Flow cytometry was used to detect the effects of astragalus polysaccharide on the cell cycle and apoptosis of Hep G2.215. Results The CCK-8 assay experiments showed that when Astragalus polysaccharides concentration was 1000 μg/ml, it had an inhibitory effect on the growth of Hep G2.215 cells, and the difference was statistically significant compared with the control group(P<0.05).The plat cell cloning experiments showed that a low concentration of Astragalus polysaccharides(100 μg/ml) could inhibit the cloning of Hep G2.215 cells, and the difference was statistically significant compared with the control group(P <0.05). Flow Cytometry results showed that the proportion of cells in the G2/M phase decreased compared with the control group(P <0.05), and the apoptosis rate of Hep G2.215 cells treated by Astragalus polysaccharides was higher than that of the control group(P <0.05). Conclusions The Astragalus polysaccharides can inhibit the growth of hepatocellular carcinoma Hep G2.215 cells, and the mechanism may be that Astragalus polysaccharides block Hep G2.215cells from entering the G2/M phase, activate the apoptosis system and induce apoptosis.