电化学检测SARS-CoV-2的无机及分析化学综合教学实验

Comprehensive Teaching Experiment of Inorganic and Analytical Chemistry for Electrochemical Detection of SARS-COV-2

实验将熵驱动DNA链置换和SARS-CoV-2检测等前沿科研引入到本科化学专业实验教学中。在热力学熵驱动下,SARS-CoV-2的靶标核酸与电极上双链固定探针上的Toehold区域结合并引发链置换反应,将一条亚甲基蓝修饰的信号探针从电极表面上置换下来,使得电流信号减弱。根据电流信号强度变化与SARS-CoV-2靶标核酸浓度的关系,实现对SARS-CoV-2简单、低成本及强特异性的检测。实验综合应用无机及分析化学基础知识,实验操作难度适中,内容新颖,开阔学生学术视野的同时掌握科学系统的研究方法。

In this comprehensive design experiment,entropy-driven DNA strand displacement reaction and SARS-CoV-2 detection are introduced into the experimental teaching of undergraduate chemistry major.Upon hybridizing with the toehold region,the target nucleic acid of SARS-CoV-2 binds to one end of the template on the electrode and displaces the methylene blue(MB)-modified signal probe driven thermodynamically by entropy.During the signal probe is far away from the electrode surface,the current signal decreases.Based on the relationship between current intensity and target nucleic acid concentration,this method realizes simple,low-cost and highly specific detection of SARS-CoV-2.This experiment comprehensively applies the basic knowledge of inorganic and analytical chemistry.Moreover,moderate experimental operation and novel content help students broaden their horizons and to learn a research approach systematic science.