摘要
以甘薯近缘野生种Ipomoea trifida根尖为材料,分析0.1%秋水仙素、对二氯苯饱和溶液、2 mmol/L的8-羟基喹啉三种试剂不同处理时间对细胞有丝分裂指数、制片效果的影响。结果表明,对二氯苯饱和溶液对甘薯野生种I.trifida根尖进行3 h处理,细胞有丝分裂指数最高,染色体制片效果较好,为最佳预处理方法。基于上述体系鉴定了本试验中的甘薯野生种I.trifida为二倍体(2 n=2 x=30),秋水仙素加倍处理后的I.trifid是四倍体(2 n=4 x=60),徐薯18是六倍体(2 n=6 x=90)。
Using the root tips of the closely related wild species Ipomoea trifida as materials.The three reagents including 0.1%colchicine,p-dichlorobenzene saturated solution and 2 mmol/L 8-hydroxyquinoline were used to analyze the effects on slicing of root tip in different treatment time,the root tips of Ipomoea trifida from a wild relative species of sweet potato.The highest mitotic index and the optimal slide specimen were obtained when root tips were treated with p-dichlorobenzene saturated solution for 3 hours.Based on the above system,the wild species of sweet potato I.trifida was idendified as diploid,I.trifida identified as tetraploid after colchicine doubling,Xinshu 18 identified as hexaploid.
作者
吉士东
王丽
胡启国
杨爱梅
JI Shidong;WANG Li;HU Qiguo;YANG Aimei(College of Life Science and College of Agricultural Engineering,Nanyang Normal University,Nanyang He’nan 473061,China;Shangqiu Academy of Agriculture and Forestry Sciences,Shangqiu He’nan 476000,China)
出处
《种子》
北大核心
2022年第4期121-125,共5页
Seed
基金
河南省高等学校重点科研项目(16 B 180002)
南阳师范学院博士专项(70351、15076)
河南省高等学校大学生创新训练计划项目(202010481033)。
关键词
甘薯野生种
染色体数
秋水仙素
对二氯苯
8-羟基喹啉
wild species of sweet potato
chromosome number
colchicine
p-dichlorobenzene
8-hydroxyquinoline