化瘀消癥颗粒调控Talin1抑制人绒毛膜滋养层细胞侵袭迁移的研究

The Invasion and Migration Effects of Huayu Xiaozheng Granules on Human Chorionic Trophoblast Cells through Regulating Talin1

目的研究化瘀消癥颗粒含药血清对人绒毛膜滋养层细胞HTR-8/SVneo增殖、侵袭和迁移的影响,并探讨其作用机制。方法制备化瘀消癥颗粒含药血清,CCK8实验检测不同浓度化瘀消癥颗粒含药血清对HTR-8/SVneo细胞增殖的影响并筛选后续实验药物浓度,划痕实验、Transwell实验检测化瘀消癥颗粒含药血清对HTR-8/SVneo细胞侵袭、迁移的影响;qPCR及Western blot检测HTR-8/SVneo细胞中Talin1、MMP-2、N-cadherin、Snail、Rap1GAP、ITGB3的mRNA和蛋白表达。结果化瘀消癥颗粒含药血清可抑制HTR-8/SVneo细胞的增殖、侵袭和迁移;与空白对照组比较,化瘀消癥颗粒含药血清可下调侵袭相关因子Talin1、MMP-2、N-cadherin、Snail、Rap1GAP、ITGB3的mRNA和蛋白表达(P<0.05,P<0.01)。结论化瘀消癥颗粒含药血清可能通过调控Rap1/Talin1/ITGB3通路而下调Talin1表达来发挥杀胚作用。

OBJECTIVE To study the effects of Huayu Xiaozheng Granules containing serum on the proliferation,invasion and migration of HTR-8/SVneo cells,and to explore its mechanism.METHODS Huayu Xiaozheng Granules containing serum was prepared,and CCK8 test was conducted to detect the effects of different concentrations and doses of Huayu Xiaozheng Granules containing serum on HTR-8/SVneo cell proliferation,and to screen the optimal concentration and dose of subsequent experimental drugs.Scratch test and Transwell test were used to detect the effects of Huayu Xiaozheng Granules containing serum on the invasion and migration of HTR-8/SVneo cells.The mRNA and protein expressions of Talin1,MMP-2,N-cadherin and Snail and the pathway proteins Rap1GAP and ITGB3 in HTR-8/SVneo cells were detected by qPCR and Western blot.RESULTS Huayu Xiaozheng Granules containing serum inhibited the proliferation,invasion and migration of HTR-8/SVneo cells.Compared with blank control group,Huayu Xiaozheng Granules containing serum down-regulated the expressions of invasion related factors Talin1,MMP-2,N-cadherin and Snail(P<0.05,P<0.01)and the expressions of pathway proteins Rap1GAP and ITGB3 in HTR-8/SVneo cells(P<0.05,P<0.01).CONCLUSION Huayu Xiaozheng Granules containing serum can down-regulate the expression of Talin1 through regulating Rap1/Talin1/ITGB3 signaling pathway.