HPV16/18 E6和TRIM25蛋白在不同宫颈组织中的表达及意义

Expression and Clinical Significance of HPV16/18 E6 and TRIM25 Proteins in Different Cervical Tissues

目的研究不同宫颈组织中HPV16/18 E6和TRIM25蛋白表达水平及意义。方法选取宫颈癌患者105例、CINIⅠ~Ⅲ患者62例、CINⅠ患者58例和慢性宫颈炎患者50例,免疫组织化学方法检测在各宫颈组织中HPV16/18 E6和TRIM25的表达情况。用Spearman相关分析法分析HPV16/18 E6和TRIM25表达在宫颈癌组织中的相关性。用Kaplan-Meier法分析HPV16/18 E6和TRIM25表达水平与宫颈癌患者预后的关系;COX回归分析法分析宫颈癌患者预后的危险因素。结果HPV16/18 E6、TRIM25在不同宫颈组织中表达比较,差异有统计学意义。HPV16/18 E6和TRIM25在宫颈癌组织中表达水平呈负相关。HPV16/18 E6表达与组织分化程度、淋巴结转移、肿瘤直径有关,TRIM25表达与组织分化程度、淋巴结转移有关。HPV16/18 E6阳性表达患者的总生存期短于阴性表达患者。TRIM25阴性表达患者的总生存期短于阳性表达患者。淋巴结转移、HPV16/18 E6可作为宫颈癌患者预后的独立危险因素,TRIM25可作为宫颈癌患者预后的独立保护因素。结论HPV16/18 E6、TRIM25表达与不同宫颈病变的发生、发展及预后有关,有望成为HPV感染及宫颈癌辅助基因治疗的靶点。

Objective To observe the expression changes of HPV16/18 E6 and TRIM25 in different cervical tissues and their significance. Methods A total of 105 patients with cervical cancer, 62 patients with CIN Ⅱ~Ⅲ, 58 patients with CINI and 50 patients with chronic cervicitis were selected to detect the expression of HPV16/18 E6 and TRIM25 in cervical tissues by immunohistochemistry. Spearman correlation analysis was used to analyze the correlation between the two factors. Kaplan-meier method was used to analyze the relationship between HPV16/18 E6 and TRIM25 expression levels and survival of cervical cancer patients. COX proportional hazard model to explore the prognostic risk factors of patients. Results The expression of HPV16/18 E6 and TRIM25 in different cervical tissues was significantly different. HPV16/18 E6 and TRIM25 expression levels were negatively correlated in cervical cancer tissues. The expression of HPV16/18 E6 was related to the degree of tissue differentiation, lymph node metastasis and tumor size, and TRIM25 was related to the degree of tissue differentiation and lymph node metastasis. The overall survival of patients with positive HPV16/18 E6 expression was shorter than that of patients with negative HPV16/18 E6 expression. The overall survival of patients with negative expression of TRIM25 was shorter than that of patients with positive expression. Lymph node metastasis and HPV16/18 E6 could be independent risk factors, and TRIM25 could be independent protective factors for prognosis of cervical cancer patients. Conclusion The expression of HPV16/18 E6 and TRIM25 is related to the occurrence, development and prognosis of different cervical lesions, which is expected to be the target of HPV infection and cervical cancer assisted gene therapy.