病毒性毛细支气管炎患儿血清白细胞介素-6、酪氨酸激酶/信号传导及转录激活因子信号通路与免疫功能的关系

Correlations of serum interleukin-6, Janus kinase/signal transducers and activators of transcription protein with immune function in children with viral bronchiolitis

目的探讨病毒性毛细支气管炎患儿外周血清白细胞介素-6(IL-6)水平、酪氨酸激酶/信号传导及转录激活因子(JAK/STAT)信号通路及免疫功能的变化。方法选择首次确诊为病毒性毛细支气管炎的112例患儿为研究对象,包括轻度50例、中度40例和重度22例,另外选择30例健康儿童作为对照组。采用酶联免疫吸附法(ELISA)检测血清IL-6,Western blot法检测外周血单个核细胞(PBMCs)中磷酸化酪氨酸激酶(p-JAK)和磷酸化信号转导激活转录因子(p-STAT)蛋白相对表达量,免疫透射比浊法检测血清免疫球蛋白(Ig)A、IgG和IgM,流式细胞术检测CD4^(+)和CD8^(+)百分比。结果与对照组比较,轻中度患儿IL-6、p-JAK、p-STAT升高,而IgA、IgG和IgM、CD4^(+)和CD8^(+)百分比降低,差异有统计学意义(P<0.05);重度患儿IL-6、p-JAK和p-STAT高于轻中度患儿,IgA、IgG和IgM、CD4^(+)和CD8^(+)百分比低于轻中度患儿,差异有统计学意义(P<0.05);轻中度患儿上述指标比较,差异无统计学意义(P>0.05)。结论IL-6高表达和JAK/STAT信号通路激活可能参与了病毒性毛细支气管炎的发生和发展,并与患儿的体液和细胞免疫功能紊乱密切相关,可为疾病治疗的新靶点提供理论依据。

Objective To explore levels of serum interleukin-6(IL-6),Janus kinase/signal transducers and activators of transcription(JAK/STAT)signal pathway and changes of immune function in children with viral bronchiolitis.Methods A total of 112 children initially diagnosed with viral bronchiolitis were chosen as study objects,including 50 mild children,40 moderate children and 22 severe children,anotheR30 healthy children were selected as control group.Serum IL-6 was detected by enzyme-linked immunosorbent assay(ELISA),relative expressions of phosphorylated JAK(p-JAK)and phosphorylated STAT(p-STAT)protein in peripheral blood mononuclear cells(PBMCs)were detected by Western blot,serum immune globulin(Ig)A,IgG and IgM were detected by immunoturbidimetry,and percentages of CD4^(+)and CD8^(+)were detected by flow cytometry.Results Compared with the control group,the levels of IL-6,p-JAK and p-STAT in mild and moderate children were significantly increased,IgA,IgG and IgM,CD4^(+)and CD8^(+)percentage were significantly decreased(P<0.05);IL-6,p-JAK and p-STAT in severe viral bronchiolitis children were significantly higher,and IgA,IgG and IgM,CD4^(+)and CD8^(+)percentages were significantly lower than those in mild and moderate children(P<0.05);there were no differences of the above indexes in mild and moderate viral bronchiolitis children(P>0.05).Conclusion Higher expression of IL-6 and activation of JAK/STAT signal pathway may be involved in the occurrence and development of viral bronchiolitis,and are closely related to disorder of humoral and cellular immune function in children,which can provide a theoretical basis for new target of disease treatment.