乳酸菌发酵提取物保护皮肤氧化损伤的作用与机理

Effect and mechanism of lactic acid bacteria fermentation extracts to protect skin from oxidative stress damage

通过发酵培养获得了5种乳酸菌发酵提取物:植物乳杆菌发酵提取物(FE1)、类干酪乳杆菌发酵提取物(FE2)、嗜酸乳杆菌发酵提取物(FE3)、高加索酸奶乳杆菌发酵提取物(FE4)、瑞士乳杆菌发酵提取物(FE5)。从生化、细胞、分子3种水平对所得提取物的抗氧化功效和保护细胞氧化应激损伤的效果和机制进行检测。质量浓度为10 g/L FE1清除自由基的能力最强,可清除49.14%的·OH和73.99%的·O_(2)^(–)。质量浓度为10 g/L的FE1、FE2和FE3能够显著提高氧化应激损伤条件下人皮肤成纤维细胞的存活率与谷胱甘肽过氧化物酶活性,降低细胞内活性氧含量。FE1保护下胞内过氧化氢酶、超氧化物歧化酶活性及总抗氧化能力显著提升。FE1通过促进Sirt1以及Wnt/β-catenin通路中β-catenin、SMP30、FoxO3a、Tbx3的表达以提升抗氧化能力。结果表明,FE1良好的抗氧化功效能够减缓H_(2)O_(2)刺激引起皮肤细胞的氧化损伤。

Five kinds of lactic acid bacteria fermentation extracts,Lactobacillus plantarum fermentation extract(FE1),Lactobacillus paracasei fermentation extract(FE2),Lactobacillus acidophilus fermentation extract(FE3),Lactobacillus kefiri fermentation extract(FE4)and Lactobacillus helveticus fermentation extract(FE5)were obtained by fermentation culture.The antioxidant effect and mechanism of protecting cells from oxidative stress damage of the obtained extracts were tested at biochemical,cellular,and molecular levels.Mass concentration of 10 g/L FE1 had the strongest ability to scavenge free radicals,which could scavenge 49.14%of·OH and 73.99%of·O_(2)^(-).Mass concentration of 10 g/L FE1,FE2 and FE3could significantly improve the survival rate and glutathione peroxidase activity of human skin fibroblasts and reduce the content of reactive oxygen species in cells under oxidative stress damage conditions.Under the protection of FE1,the activities of intracellular catalase,superoxide dismutase and total antioxidant capacity were significantly increased.FE1 enhanced the antioxidant capacity by upregulating the expression of Sirt1 and genes in Wnt/β-catenin pathways includingβ-catenin,SMP30,FoxO3a,and Tbx3.The above results indicated that the good antioxidant effect of FE1 could slow down the oxidative stress damage of skin cells caused by H_(2)O_(2).