小茴香油微乳凝胶的制备及其体外透皮促渗行为研究

Research on Preparation of Fennel Oil Microemulsion and Promotion of Transdermal Permeability in Vitro

目的优化筛选出小茴香油微乳凝胶的最佳处方组成,并考察小茴香油微乳凝胶的体外透皮性能。方法采用星点设计效应面法分别优化了微乳和微乳凝胶的处方,Franz扩散池法考察小茴香油微乳及其凝胶的体外透皮性能。结果微乳最优处方为油相∶乳化剂和助乳化剂(吐温-80︰二乙二醇单乙基醚=2.5︰1)∶水=20︰27.6︰52.4,进一步制备得到了小茴香油微乳凝胶。小茴香油微乳的流动性及稳定性好,平均粒径为(42.03±0.9)nm、多分散性指数为(0.154±0.005)。小茴香油微乳凝胶均匀细腻,易涂展,有一定流动性,为乳白色半透明半固体凝胶。体外透皮性能结果表明小茴香油作为油相,制备成微乳和微乳凝胶,其体外透皮吸收量分别提高了3,4倍。结论该研究为小茴香油的制剂技术奠定了基础,也为挥发油类药物的广泛应用提供了理论依据。

OBJECTIVE To optimize the optimal formulation composition of fennel oil microemulsion gel and to investigate its transdermal performance in vitro.METHODS The formulations of microemulsion and microemulsion gel were optimized by central composite design-response surface method,and the transdermal properties of the microemulsion and its gel were investigated by Franz diffusion pool method.RESULTS The optimal formula of microemulsion was oil phase∶emulsifier and co-emulsifier(Tween-80∶diethylene glycol monoethyl ether=2.5∶1)∶water=20∶27.6∶52.4,and the microemulsion gel of fennel oil was prepared.The microemulsion of fennel oil had good fluidity and stability,the average particle size was(42.03±0.9)nm,and the polydispersion coefficient(PDI)was(0.154±0.005).The obtained microemulsion gel of fennel oil was uniform and delicate,easy to spread,and had a certain fluidity.It was an opalescent translucent semi-solid gel.The results of in vitro transdermal performance showed that microemulsion and microemulsion gel were prepared from fennel oil as oil phase,and the in vitro transdermal absorption of fennel oil was increased by 3 times and 4 times respectively.CONCLUSION This study lays a foundation for the preparation technology of fennel oil and provides a theoretical basis for the extensive application of volatile oil drugs.