NOS3单核苷酸多态性与6~13岁儿童肥胖的相关性

Association of the single nucleotide polymorphism of NOS3 gene with obesity in children aged 6~13 years

目的 探讨NOS3基因rs1799983与rs2566508多态性与6~13岁儿童肥胖的相关性,为进一步探索肥胖的发生、发展、预防和治疗提供科学依据。方法 2019年9月在新疆沙湾市某小学进行横断面调查,按照纳入与排除标准,筛选出肥胖组和对照组儿童各100例,其中汉族儿童122例,哈萨克族儿童78例。采用芯片捕获测序技术、高通量测序技术检测NOS3基因rs1799983、rs2566508多态性,分析与汉族、哈萨克族儿童肥胖的相关性。结果 1)NOS3基因rs2566508多态性与汉族6~13岁女童的肥胖发生可能相关,T、G等位基因分布频率在对照组与肥胖组间差异有统计学意义(χ^(2)=11.247,P=0.001),G等位基因携带者发生肥胖风险显著升高(OR=3.889,95%CI:1.707~8.860),TT、TG、GG基因型分布频率在两组间差异也有统计学意义(χ^(2)=10.389,P=0.004)。但在汉族男童以及哈萨克族男童、女童中未发现rs2566508多态性与肥胖的相关性。2)NOS3基因rs1799983等位基因和基因型分布频率在汉族和哈萨克族儿童中组间差异无统计学意义(P>0.05)。结论 NOS3基因rs2566508多态性与汉族6~13岁女童肥胖的发生可能相关,且G等位基因携带者发生肥胖风险显著升高。

Objective To analyze the correlation of polymorphisms of NOS3 rs1799983, rs2566508 with obesity in children aged 6 to 13, in order to provide scientific basis for further exploration of obesity occurrence, development, prevention and treatment. Methods A cross-sectional survey was conducted at a primary school in Shawan City, Xinjiang Province in September 2019. According to the inclusion and exclusion criteria, 100 cases were screened with obesity and 100 children were in control group. There were 122 cases of Han nationality and 78 cases of Kazakh. Chip capture sequencing and high-throughput sequencing technology were used to detect NOS3 gene rs1799983, rs2566508 polymorphism. Then their correlations with obesity among Han and Kazakh children were analyzed. Results 1) NOS3 gene rs2566508 polymorphism may be associated with obesity in Han girls aged 6 to 13 years. There were significant differences in the frequencies of T and G alleles distribution between the control group and obesity group(χ^(2)=11.247, P=0.001). The risk of obesity increased significantly in carriers of G allele(OR=3.889, 95%CI:1.707-8.860). There were also significant differences in the frequencies of TT, TG and GG genotypes distribution between the two groups(χ^(2)=10.389, P=0.004). However, no association between rs2566508 polymorphism and obesity was found in Han boys as well as Kazakh boys and girls. 2) The allele and genotype distribution frequencies of NOS3 gene rs1799983 were not statistically significant in the group differences between Han and Kazakh children(P>0.05). Conclusion NOS3 gene rs2566508 polymorphism may be associated with obesity in Han girls aged 6 to 13, and the risk of obesity increases significantly in the carriers of G allele.