抗消化淀粉体外的评价方法

Establishment of the Evaluation of Resistant Starch

采用体外模拟方法,研究了胃蛋白酶处理条件、a 淀粉酶消化条件等对抗消化淀粉含量测定的影响.结果表明,用2%胃蛋白酶在36℃条件下对淀粉样品处理70min,可有效除去淀粉中的蛋白质组分.当淀粉酶处于最高活性条件下,pH值为6.3～7,作用时间为20min,可以模拟体内淀粉酶对淀粉的消化过程,基本上除去了可消化淀粉部分,剩余部分即为抗消化淀粉.

The evaluation of starch in vitro is investigated by the first treatment of starch with pepsin at different pepsin contents, pH values and reaction time to remove protein component, and then the reaction of amylase with the treated starch at various pH values and reaction time is studied. The results show that 2 % content of pepsin can almost completely remove the proteins in starch by reacting with the starch sample for 70 minutes. The artificial conditions like pH=6.3～7, reaction time of 20 minutes,can simulate the reaction of αamylase with starch in vivo,and hydrolyze the non-resistant starch molecules to comparatively small molecules with different lengthes, and the remains are considered as the resistant starch.