光强调制型SPRi生物传感器的应用及分析方法

Application and Analysis Method of Light Intensity Modulated SPRi Biosensor

表面等离体激元共振是一种在生物化学、生物分析化学和生物医学等领域中得到广泛应用的光学传感技术,因其高通量、无需标记、高灵敏度等优点受到越来越广泛的重视。该研究通过分析SPRi(SPRi maging)传感器对不同折射率的样品与共振角的检测规律,得出系统应用于样品折射率、生物膜样品厚度分布及覆盖面密度检测的方法;从两种物质相互作用的平衡反应原理推导、分析了SPRi传感器进行实时分子相互作用检测的方法,为SPRi传感器的应用实验提供借鉴。

Surface plasmon resonance is an optical sensing technology widely used in biochemistry,bioanalytical chemistry and biomedicine and other fields.It has attracted more and more attention because of its high throughput,no marking required and high sensitivity.By analyzing the detection law of SPRi(SPRi maging)sensor for samples with different refractive index and resonance angle,this study obtains the method applied to the detection of sample refractive index,biofilm sample thickness distribution and coverage density;based on the equilibrium reaction principle of the interaction between two substances,the method of real-time molecular interaction detection by SPRi sensor is deduced and analyzed,which provides reference for the application experiment of SPRi sensor.