雷公藤中倍半萜生物碱类化学成分的研究

Chemical constituents of sesquiterpene alkaloids from Tripterygium wilfordii

目的对雷公藤Tripterygium wilfordii乙醇提取物氯仿部位中的倍半萜生物碱类成分进行研究。方法采用酸提碱沉法,结合中性氧化铝、十八烷基硅烷键合硅胶(ODS)及半制备高效液相等多种色谱技术进行分离纯化,并运用1D-NMR、2D-NMR、MS、ECD等波谱学方法对化合物进行结构鉴定。结果从雷公藤乙醇提取物氯仿部位中分离得到5个倍半萜生物碱类成分,分别鉴定为(1S,4S,5R,6R,7R,8S,9S,10S)-1,8,11-triacetoxy-7-nicotinoyloxy-4,5-dihydroxy-dihydroagarofuran(1)、triptersinine U(2)、macroregeline F(3)、雷公藤宁碱B(4)和triptonine B(5)。结论化合物1为新化合物,命名为雷公藤司宁Z15;化合物3为首次从该植物中分离得到。

Objective To study the sesquiterpene alkaloids from the chloroform fraction of the ethanolic extract of Tripterygium wilfordii.Methods Compounds were isolated and purified by acid extraction and alkaline precipitation method,combined with various chromatographic techniques such as neutral alumina,octadecylsilane bonded silica(ODS)and semi-preparative high performance liquid chromatography.Their structures were elucidated by means of various spectroscopic techniques such as 1 D-NMR,2 D-NMR,MS,and ECD.Results A total of five sesquiterpene alkaloids were isolated from the chloroform fraction of the ethanolic extract of T.wilfordii,and identified as(1 S,4 S,5 R,6 R,7 R,8 S,9 S,10 S)-1,8,11-triacetoxy-7-nicotinoyloxy-4,5-dihydroxy-dihydroagarofuran(1),triptersinine U(2),macroregeline F(3),wilfornine B(4),and triptonine B(5).Conclusion Compound 1 is a new sesquiterpene alkaloid named triptersinine Z15,and compound 3 is isolated from T.wilfordii for the first time.