萱草叶片响应低温胁迫的转录组分析

Transcriptome Analysis of Hemerocallis fulva Leaves Respond to Low Temperature Stress

低温胁迫是萱草(Hemerocallis fulva)生长过程中经常会遭遇的一种非生物胁迫。比较了萱草叶片在低温处理(10、5、0℃)下转录组与对照(15℃)数据的差异,共筛选出差异表达基因2457个,其中上调基因1253个,下调基因1204个。差异表达基因主要富集在细胞过程、代谢过程和催化活性等49个GO过程,代谢途径、次生代谢产物的生物合成、植物激素信号转导等42条KEGG代谢途径中。其中参与植物激素信号转导通路的差异表达基因发生了不同程度的变化,GH3.10基因上调至对照组的13.624倍,IAA1基因下调0.120倍;参与可溶性糖合成通路的差异基因发生了0.076~28.114倍不同程度的变化。随后对3个低温处理组共有的29个差异表达基因进行热图和网络调控分析,基于基因在网络调控中的位置,对ABCF5、OFPs和SWEETs等基因在冷应答的作用进行了分析。本研究结果为进一步挖掘萱草低温响应的关键基因及耐寒萱草种质开发、分子育种提供了一定的理论支撑。

Low temperature is a kind of abiotic stress in the growth process of Hemerocallis fulva.Thetranscriptome data of Hemerocallis fulva leaves under cold treatment groups(10,5,0℃)and control group(15℃)was compared,and a total of 2457 DEGs were screened,including 1253 up-regulated genes and 1204 down-regulated genes.The results showed that differentially expressed genes(DEGs)were mainly concentratedin 49 GO processes such as cellular process,metabolic process and catalytic activity,and 42 KEGG metabolicpathways,including metabolic pathways,biosynthesis of secondary metabolites and plant hormone signaltransduction.Among them,the DEGs involved in plant hormone signal transduction pathway changed in varyingdegrees,GH3.10 gene was increased to 13.624 times higher than the control group,IAA1 gene was downregulated to 0.120 times;the DEGs involved in soluble sugar synthesis pathway also changed by 0.076-28.114 times.Then,29 DEGs shared by treatment groups were analyzed by heat map and gene regulation networks.Based on the position of genes in gene regulation networks,the roles of ABCF5,OFPs and SWEETs in responseto cold were analyzed respectively.The results of this study provide a theoretical basis for further exploring thekey genes of low temperature response,germplasm development and molecular breeding of Hemerocallis fulva.