摘要
利用Illumina高通量测序平台对苦马豆(Sphaerophysa salsula)进行测序和组装,获得完整的苦马豆叶绿体基因组序列。组装结果表明,苦马豆叶绿体基因组全长123327 bp,存在IR区丢失,不具有四分体结构;注释结果显示,苦马豆叶绿体基因组共编码108个基因,其中包含74个蛋白编码基因、4个rRNA基因和30个tRNA基因;叶绿体基因组序列上共检测到99个SSR位点,包含75个单核苷酸、17个二核苷酸和7个三核苷酸重复单元;系统发育分析显示,苦马豆和骆驼刺为姊妹群,亲缘关系最近。这为今后苦马豆遗传多样性、群体遗传结构和物种形成机制研究奠定了基础。
The whole chloroplast genome of Sphaerophysa salsula was sequenced and assembled based onIllumina high-throughput sequencing platform.The assembly result indicated that the whole chloroplast genomeof S.salsula had a total length of 123327 bp,with a loss of IR region.The chloroplast genome encoded a total of108 genes,including 74 protein-coding genes,4 r RNA genes and 30 t RNA genes.and 99 SSR loci were found,and among them the number of mononucleotide,dinucleotide and trinucleotide repeat motifs was 75,17 and 7,respectively.The phylogenetic analysis results showed that S.salsula and Alhagi sparsifolia were sister species,which had the closest genetic relationship.The results would lay the foundation for the genetic diversity,population genetic structure and speciation mechanism of S.salsula in the future.
作者
苏丹丹
刘玉萍
刘涛
郑长远
张雨
王亚男
秦娜
苏旭
SU Dandan;LIU Yuping;LIU Tao;ZHENG Changyuan;ZHANG Yu;WANG Yanan;QIN Na;SU Xu(School of Life Science,Qinghai Normal University,Xining 810008;Key Laboratory of Medicinal Animal and Plant Resources of the Qing‐hai-Tibet Plateau in Qinghai Province,Qinghai Normal University,Xining 810008;Academy of Plateau Science and Sustainability,Qinghai Normal University,Xining 810016)
出处
《植物研究》
CAS
CSCD
北大核心
2022年第3期446-454,共9页
Bulletin of Botanical Research
基金
科技基础资源调查专项项目(2017FY100202-02)
祁连山国家公园青海研究中心开放课题
青海省青藏高原药用动植物资源重点实验室项目(2020-ZJ-Y40)。
