石斛酚对大鼠急性心肌梗死保护作用的机制研究

Study on the mechanism of the protective effects of gigantol on the rats with acute myocardial infarction

为了探讨石斛酚对大鼠急性心肌梗死(AMI)的治疗作用及其可能机制,文章采用结扎大鼠冠状动脉前降支,建立AMI模型,存活者随机分为模型组和石斛酚治疗组,另外设立假手术组,并测定各组心肌梗死范围(MIS)、磷酸肌酸激酶(CPK)活性及炎症因子表达,同时测定各组缺血区心肌组织核转录因子-kappaB(NF-κB)的表达变化。结果显示,石斛酚能显著降低AMI大鼠MIS、血清CPK活性及炎症因子的表达,且抑制NF-κB的激活。石斛酚对AMI大鼠的保护作用机制可能与抑制NF-κB激活有关。

To investigate the protective effects of gigantol on the rats with acute myocardial infarction(AMI), the anterior descending coronary artery of rats was ligated to establish the model of AMI in this paper. The survivors were randomly divided into model group and gigantol treatment group, with a sham operation group. We examined the myocardial infarct size(MIS), serum creatine phosphokinase(CPK) activity and the expression of inflammatory factors of each group. Additionally, we also studied the changes of nuclear transcription factor-kappaB(NF-κB) in the ischemic myocardial tissue. The results showed that the gigantol reduced MIS, serum CPK and the expression of inflammatory factors, with the inhibition of NF-κB activation. The protective effects of gigantol on the rats with AMI may be related to its inhibition of NF-κB activation.