蒙药扎冲十三味丸对脑缺血大鼠神经行为功能的影响

Effect of Mongolian medicine Zhachong 13 Pill on neurobehavioral function of rats with cerebral ischemia

目的 探讨蒙药扎冲十三味丸对脑缺血大鼠神经功能的影响。方法 SD大鼠随机分为假手术组、模型组、尼莫地平(阳性对照,0.02 g·kg^(-1))组和扎冲十三味丸高、中、低剂量(0.24、0.12、0.06 g·kg^(-1))组,除假手术组外,其余组大鼠采用ip硝普钠2 mL·kg^(-1)合并双侧颈总动脉结扎制备脑缺血模型,于术前2 h给药1次,术后连续给药5 d。术后24、72、120 h进行神经病学症状评分、平衡木行走实验、肌力评定实验和旷场实验。结果 与模型组比较,在术后24 h,扎冲十三味丸高、中剂量组和尼莫地平组神经病学症状评分显著降低(P<0.05);在术后72、120 h,扎冲十三味丸高、中、低剂量组和尼莫地平组神经病学症状评分均显著降低(P<0.05)。与模型组比较,扎冲十三味丸高、中剂量组和尼莫地平组在术后72和120 h平衡木行走评分均显著降低(P<0.05)。与模型组比较,术后72、120 h,扎冲十三味丸高剂量组大鼠肌力评分显著升高(P<0.05)。与模型组比较,扎冲十三味丸高、中、低剂量组和尼莫地平组各时相大鼠跨格次数显著减少(P<0.05);扎冲十三味丸高剂量组和尼莫地平组各时相大鼠站立次数显著减少(P<0.05),扎冲十三味丸中剂量术后24、120 h以及低剂量组术后120 h大鼠站立次数均显著减少(P<0.05)。结论 扎冲十三味丸可改善脑缺血神经功能障碍,改善受损大鼠运动失调、偏瘫和焦虑情绪。

Objective To investigate the effect of Mongolian medicine Zhachong 13 Pill on neural function in rats with cerebral ischemia.Methods SD rats were randomly divided into sham operation group,model group,Zhachong 13 Pill high,medium and low-dose(0.24,0.12 and 0.06 g·kg^(-1)) group and nimodipine(positive control,0.02 g·kg^(-1)) group.The other groups were treated with ip sodium nitroprusside 2 mL·kg^(-1) combined with bilateral common carotid artery ligation to prepare cerebral ischemia model.The rats were administered once 2 h before surgery and continuously for 5 d after surgery.Neurological symptom score,balance beam walking test,muscle strength assessment test and open field test were performed at 24,72 and 120 h postoperatively.Results Compared with model group,24 h after surgery,neurological score of Zhachong 13 Pill high and medium dose groups and nimodipine group was significantly decreased(P < 0.05).At 72 and 120 h postoperatively,neurological scores in Zhachong 13 Pill high,medium and low-dose groups and nimodipine group were significantly decreased(P < 0.05).Compared with model group,balance beam walking score in Zhachong 13 Pill high and medium dose groups and nimodipine group was significantly decreased at72 and 120 h after surgery(P < 0.05).Compared with model group,muscle strength score of Zhachong 13 Pill high group was significantly increased at 72 and 120 h after surgery(P < 0.05).Compared with model group,the number of translatations in Zhachong 13 Pill high,medium and low dose groups and nimodipine groups were significantly decreased(P < 0.05).The standing times of Zhachong 13 Pill high dose group and nimodipine group were significantly decreased at each time phase(P < 0.05),and the standing times of Zhachong 13 Pill medium dose group and low dose group were significantly decreased at 24,120 h after surgery(P < 0.05).Conclusion Zhachong 13 Pill can improve the neurological dysfunction of cerebral ischemia,dyskinesia,hemiplegia and anxiety in rats.