摘要
荧光分子探针已成为当代化学生物学和生物医药领域不可或缺的研究工具,被成功应用于活细胞内重要生物物种的动态可视化和人类重大疾病的早期诊治研究.本文总结了包括阴、阳离子,活性氧、氮、碳、硫物种和酶在内的重要生物标志物在疾病发生发展中的生物学功能,以及细胞和活体层次靶向可视化这些标志物的荧光分子探针前沿进展,归纳了现有探针体系所面临的问题和挑战,并展望了该领域的发展方向.
Fluorescent probes have emerged as indispensable chemical tools to the field of chemical biology and medicine.The ability to detect intracellular species and monitor physiological processes has not only advanced our knowledge in biology but has provided new approaches towards disease diagnosis.In this review,we detail the design criteria and strategies for some recently reported fluorescent probes that can detect a wide range of biologically important species in cells and in vivo.In doing so,we highlight the importance of each biological species and their role in biological systems and for disease progression.We then discuss the current problems and challenges of existing technologies and provide our perspective on the future directions of the research area.Overall,we hope this review will provide inspiration for researchers and prove as useful guide for the development of the next generation of fluorescent probes.
作者
豆伟涛
韩海浩
Adam C.Sedgwick
朱国彪
臧奕
杨欣荣
Juyoung Yoon
Tony DJames
李佳
贺晓鹏
Wei-Tao Dou;Hai-Hao Han;Adam C.Sedgwick;Guo-Biao Zhu;Yi Zang;Xin-Rong Yang;Juyoung Yoon;Tony D.James;Jia Li;Xiao-Peng He(Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering,Feringa Nobel Prize Scientist Joint Research Center,Frontiers Center for Materiobiology and Dynamic Chemistry,School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai 200237,China;National Center for Drug Screening,State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China;Department of Liver Surgery&Transplantation,Liver Cancer Institute,Zhongshan Hospital,Fudan University,Key Laboratory of Carcinogenesis and Cancer Invasion,Ministry of Education,Shanghai 200032,China;Department of Chemistry,University of Bath,Bath BA27AY,UK;Department of Chemistry and Nanoscience,Ewha Womans University,Seoul 03760,Republic of Korea;Department of Chemistry,The University of Texas at Austin,Austin,TX 78712-1224,USA;School of Chemistry and Chemical Engineering,Henan Normal University,Xinxiang 453007,China)
基金
the National Natural Science Foundation of China(21788102,91853201)
the Shanghai Municipal Science and Technology Major Project(2018SHZDZX03)
the International Cooperation Program of Shanghai Science and Technology Committee(17520750100)
the Projects from the Shanghai Science and Techonology Commission(19441905000)
the Fundamental Research Funds for the Central Universities(222201717003)
the Programme of Introducing Talents of Discipline to Universities(B16017)for financial support
the National Natural Science Foundation of China(22107028)
National Postdoctoral Program for Innovative Talents(BX20190115)
Shanghai Post-doctoral Excellence Program(2019044)
China Postdoctoral Science Foundation(2020M681206)for financial support
the Project funded by China Postdoctoral Science Foundation(2020M681196)
the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering,Henan Normal University for support(2020ZD01)。
