消化系统多种癌变组织中3种蛋白质的表达谱

Expression profiles of three proteins in various cancerous tissues of digestive system

在口腔、食管和结直肠癌病灶中,TATA序列结合蛋白相关因子1(TATA-box binding protein associated factor 1 like,TAF1L)、stomatin家族蛋白1(stomatin-like protein 1,STOML1)和stomatin家族蛋白2(stomatin-like protein 2,STOML2)存在异常表达.为探究它们在消化系统癌变过程中的作用机理,利用6种消化道肿瘤(食道、胃、结肠、直肠、肝脏和胰腺)及癌旁组织的微陈列芯片,通过多重免疫组织化学染色,比对分析TAF1L、STOML1和STOML2这3种蛋白质在癌与癌旁组织的差异表达及与癌变的关联.结果发现,STOML2在食道、胃、结肠、直肠、肝脏和胰腺这6种消化系统癌组织中均呈显著性高表达(P<0.001),而同一家族成员STOML1则仅在食管癌和肝癌组织中表达明显增高(P<0.005).另外,除胰腺癌灶外,TAF1L在其余5种消化系统癌组织中也呈现高表达(P<0.005).同时,TAF1L、STOML1和STOML2这3种蛋白质均与上皮间充质转化的关键蛋白质MMP9有共定位现象.由此表明,3种蛋白质TAF1L、STOML1和STOML2在多种癌组织中可能具有广泛的促癌作用.由于3种蛋白质在多种癌组织的表达水平不同,说明它们各自存在着组织特异性,且具有不同的功能使命,需进一步研究TAF1L、STOML1和STOML2这3种蛋白质在消化系统恶性肿瘤发生发展过程中各自的分子病理学潜能,如能将它们打造成用于精准诊疗和预后判断的新型分子标志物,此研究结果将有重要的临床意义.

A variety of gastrointestinal tumors are the most malignancies with high morbidity and mortality globally.The previous studies show that there are abnormal expressions of TATA-box binding protein associated factor 1 like(TAF1L),stomatin-like protein 1(STOML1)and stomatin-like protein 2(STOML2)genes in oral,esophageal and colorectal cancer lesions.To explore the potential roles of above three genes in the carcinogenesis of digestive system,the differential expressions of TAF1L,STOML1 and STOML2 in cancerous and paracancerous tissues are compared and analyzed by multiple immunohistochemical staining using microarray of 6 cancer tissues of esophagus,stomach,liver,pancreas,colon and rectum with matched paracancerous tissues.The results show that STOML2 is significantly overexpressed in all of 6 gastrointestinal cancers(P<0.001),and STOML1,one of the same family member of STOML2,is highly expressed only in esophageal and liver cancers(P<0.005).In addition,except for pancreatic cancer,TAF1L is also abnormal highly expressed in other 5 cancer tissues(P<0.005).At the same lesion tissue section,STOML1,STOML2 and TAF1L proteins are colocated with the key protein MMP9 of epithelial-mesenchymal transformation in positive cells.This study indicates that the three proteins of TAF1L,STOML1 and STOML2 might have a wide range of cancer promoting effects in multiple cancer tissues in the digestive system.In addition,due to their different expression levels in a variety of cancer tissues,they have tissue specificity and different functional missions.Further exploration of the molecular pathological potential of TAF1L,STOML1 and STOML2 in the occurrence and development of malignant tumors in digestive system can make them serve as new molecular markers for early diagnosis,differential diagnosis,targeted therapy and prognosis prediction in multiple digestive tumors.