瑞士乳杆菌对泥鳅肠道转录组基因表达的影响

Effects of Lactobacillus helveticus on Gene Expression in Intestinal Transcriptome of Loach Misgurnus anguillicaudatus

为探究饲料添加瑞士乳杆菌HML037对泥鳅生长和抗病性基因表达的影响,分别对对照组和添加组的泥鳅肠道组织进行双端测序,获得40877个平均长度为1218 bp、N50为1942 bp的unigenes,序列比对分析后均得到注释。通过DEGseq进行基因表达差异分析,获得差异基因604个,其中326个基因显著上调,278个基因显著下调。将所有差异表达基因进行GO功能富集分析,显著富集到5条途径,其中氧化还原过程途径富集程度最显著,氧化还原酶活性途径次之,且参与这些途径的差异基因均上调。KEGG通路富集分析显示,差异基因主要富集到过氧化物酶体增殖激活受体信号通路、脂肪酸延长和脂肪消化和吸收等。本试验结果可为乳酸菌在泥鳅健康养殖中的应用提供理论支撑。

In order to explore the effect of Lactobacillus helveticus HML037 on the growth and disease resistance gene expression of loach Misgurnus anguillicaudatus,the intestinal tissues of the loach from control group(C)and L.helveticus HML037 addition group(L)were double ended sequenced by Illumina hiseq 2500 high-throughput sequencing platform.The original data were quality controlled and de novo assembled,and 40877 unigenes with an average length of 1218 bp and 1942 bp of N50.DEGseq was used to analyze gene expression difference,with 604 differential genes,of which 326 genes were significantly up-regulated and 278 genes were significantly down-regulated.All the differentially expressed genes were enriched in five pathways by GO functional enrichment analysis.The oxidation-reduction process pathway was the most abundant,followed by the oxidoreductase activity pathway,and the differentially expressed genes involved in these pathways were all up-regulated.Enrichment analysis of KEGG pathway showed that the differentially expressed genes were mainly concentrated in peroxidase proliferation-activation receptor signaling pathways,fatty acid prolongation and fat digestion and absorption.The findings provide an important reference for the improvement of intestinal growth and disease resistance performance of the loach by using L.helveticus HML037 instead of antibiotics.