Jumonji结构域蛋白3调节人牙周膜细胞的炎症反应

Jumonji Domain-containing Protein 3 Regulates the Inflammatory Response of Human Periodontal Ligament Cells

目的:研究三甲基化组蛋白H3赖氨酸K27(histone 3 lysine 27 trimethylation,H3K27me3)的去甲基化酶Jumonji结构域蛋白3(Jumonji domain-containing protein 3,Jmjd3)在人牙周膜细胞炎症反应中的调节作用。方法:分别使用牙龈卟啉单胞菌(Porphyromonas gingivalis,p.g)的脂多糖(lipopolysaccharide,LPS)和肿瘤坏死因子肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)刺激人牙周膜细胞,建立炎症模型。通过实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,Q-PCR)和免疫荧光检测Jmjd3的表达情况。后通过沉默Jmjd3,在Q-PCR和Western blot确定沉默效果后对其可能的下游靶基因进行初步筛选。结果:Jmjd3在炎症的人牙周膜细胞中表达会上调。沉默Jmjd3后,炎症介导的白细胞介素-6和白细胞介素-12b的上调受到抑制。结论:Jmjd3参与人牙周膜细胞炎症反应,并能够调节白细胞介素-6、白细胞介素-12b的表达。

Objective:To investigate the role of the histone 3 lysine 27 trimethylation(H3K27me3)demethylase Jumonji domain-containing protein 3(Jmjd3)in the regulation of the inflammatory response of human periodontal ligament cells(HPDLs).Methods:HPDLs were stimulated with lipopolysaccharide(LPS)from p.g or tumor necrosis factor-α.The expression of Jmjd3 in HPDLs was examined by quantitative real-time polymerase chain reaction(Q-PCR)and immunofluorescence.After confirmed the depletion of Jmjd3 by Q-PCR and Western blot,potential target genes were selected by silencing Jmjd3.Results:Q-PCR and immunofluorescence revealed that the expression of Jmjd3 was increased in inflamed HPDLs.Knockdown of Jmjd3 led to the suppression of inflammation-induced up-regulation of interleukin-6 and interleukin-12b.Conclusion:Jmjd3 regulated the activation of interleukin-6 and interleukin-12b in the inflammatory response of HPDLs.