穿心莲纳米乳液用于根管冲洗的清洁性实验研究

Cleaning Efficacy of Andrographis Paniculata Nanoemulsion Irrigant on Root Canals

目的:评价穿心莲纳米乳液用于根管冲洗的清洁性。方法:收集50个新鲜拔除的单根管前磨牙,随机分为5组。采用镍钛锉冠向下法预备根管,分别使用5组冲洗液进行根管冲洗。A组为穿心莲纳米乳液,B组为穿心莲提取液,C组为1%次氯酸钠(NaClO),D组为3%NaClO,E组为0.9%的生理盐水。最后每组分别用17%乙二胺四乙酸二钠(EDTA)、生理盐水冲洗根管。采用数码相机和扫描电镜观察,分别比较根尖、根中和根冠1/3区根管内碎屑百分数和玷污层评分。结果:根尖1/3:E组碎屑百分数和玷污层明显高于其他4组,C组玷污层评分明显高于A组和D组(P<0.05)。根中1/3:E组碎屑百分数和玷污层评分明显高于其他4组,C组碎屑含量明显多于A组和D组(P<0.05)。根冠1/3:E组碎屑百分数和玷污层评分明显高于其他4组,C组玷污层评分明显高于D组(P<0.05)。结论:冲洗液的清洁性排序:3%NaClO、穿心莲纳米乳液、穿心莲提取液、1%NaClO、生理盐水。穿心莲纳米乳液被认为是可能用于根管冲洗的中药冲洗液。

Objective:To evaluate the cleanliness of andrographis paniculata nanoemulsion for root canal irrigation.Methods:Fifty freshly extracted single-root canal premolars were collected and divided into five groups.During instrumentation,the nickel-titanium files,the crown-down preparation,and five irrigants were used.group A:andrographis paniculata nanoemulsion;group B:andrographis paniculata extract;group C:1%NaClO;group D:3%NaClO;and group E:0.9%saline.All groups were finally irrigated with 17%EDTA and saline,respectively.Digital camera and scanning electron microscopy were used to compare the debris and smear layer scores in the apical,middle,and coronal thirds of the root canals.Results:In the apical thirds:group E showed higher debris and smear layer score than other four groups,group C had significantly higher smear layer score than group A and D(P<0.05).In the middle thirds:group C showed significantly more debris than group A and D(P<0.05).In the coronal thirds:group C had significantly higher smear layer score than group D(P<0.05).Conclusion:The cleanliness of the irrigation fluid was ranked as follows:3%NaClO,andrographis paniculata nanoemulsion,andrographis paniculata extract,1%NaClO,and Saline.Andrographis paniculata nanoemulsion appears to be one of the Chinese medicine to be identified as a possible intracanal irrigant.