一株降解氨基甲酸乙酯酿酒酵母菌的筛选及鉴定

Screening and identification of a Saccharomyces cerevisiae strain degrading ethyl carbamate

为筛选用于葡萄酒发酵的可降解氨基甲酸乙酯(ethyl carbamate,EC)酵母菌,对酿酒葡萄、干化葡萄、酒醅进行筛选,获得7株对EC有较强降解能力的菌株,经过发酵性能、耐乙醇以及耐二氧化硫实验,发现2株菌发酵性能较好。在此基础上,对这2株菌所发酵的葡萄酒进行EC含量、理化指标、香气组分的比较及电子鼻分析,并以商业酵母XR作为对照。筛选出目的菌株,经分子鉴定后,确定该菌株为酿酒酵母,并命名为NXU12-2。研究所获得的菌株是具有氨基甲酸乙酯降解能力的酿酒酵母,其发酵的葡萄酒品质较优。该研究为葡萄酒中EC的调控提供了优质的菌种资源,具有重要意义。

In order to screen the yeast which can degrade ethyl carbamate(EC) used in wine fermentation,multiple rounds of selection with wine grapes,dried grapes,and fermented grains were adopted in this paper and seven strains with strong capacity of degrading EC were obtained.The results of fermentation performance,ethanol resistance and sulfur dioxide resistance showed that two strains had better fermentation performance.Then the contents of EC,physicochemical indexes,aroma components and electronic nose analysis of the wines fermented by the two strains were compared,and the commercial yeast XR was used as the control.After the molecular identification of the target strain,it was identified as Saccharomyces cerevisiae and was named as NXU12-2.In this study,a strain of S.cerevisiae with the ability to degrade EC was selected,and it fermented wines are of good quality.This research would provide high quality strain resources which can control EC in wine.